Guloglu M Oktar, Larsen Anna
Department of Experimental Medical Science, Faculty of Medicine, Lund University, 22184, Lund, Sweden.
Methods Mol Biol. 2016;1341:235-44. doi: 10.1007/7651_2015_235.
Human embryonic stem cells (hESCs) are a promising source for cell replacement therapies. Parkinson's disease is one of the candidate diseases for the cell replacement therapy since the motor manifestations of the disease are associated with the loss of dopaminergic neurons in the substantia nigra pars compacta. Stromal cell-derived inducing activity (SDIA) is the most commonly used method for the dopaminergic differentiation of hESCs. This chapter describes a simple, reliable, and scalable dopaminergic induction method of hESCs using PA6-derived adipocytes. Coculturing hESCs with PA6-derived adipocytes markedly reduces the variable outcomes among experiments. Moreover, the colony differentiation step of this method can also be used for the dopaminergic induction of mouse embryonic stem cells and NTERA2 cells as well.
人类胚胎干细胞（hESCs）是细胞替代疗法的一个有前景的细胞来源。帕金森病是细胞替代疗法的候选疾病之一，因为该疾病的运动表现与黑质致密部多巴胺能神经元的丧失有关。基质细胞衍生诱导活性（SDIA）是hESCs向多巴胺能分化最常用的方法。本章描述了一种使用PA6来源的脂肪细胞对hESCs进行简单、可靠且可扩展的多巴胺能诱导方法。将hESCs与PA6来源的脂肪细胞共培养可显著减少实验间的可变结果。此外，该方法的集落分化步骤也可用于小鼠胚胎干细胞和NTERA2细胞的多巴胺能诱导。
