藏红花醛促进帕金森病动物模型中多巴胺能神经元的分化和存活。
Safranal-promoted differentiation and survival of dopaminergic neurons in an animal model of Parkinson's disease.
机构信息
a Neurology Department of Xuhui Central Hospital , ShangHai , China.
出版信息
Pharm Biol. 2018 Dec;56(1):450-454. doi: 10.1080/13880209.2018.1501705.
CONTEXT
Safranal (SAF) is verified to have potential effects in promoting nerve growth.
OBJECTIVES
This study verifies the role of SAF in promoting dopaminergic neurons growth in vitro and in vivo.
MATERIAL AND METHODS
Rat neural stem cells (NSC) were treated with 1, 20, or 100 ng/mL of SAF, and the expression levels of tyrosine hydroxylase (TH) and dopamine transporter (DAT) were assayed by flow cytometry and real-time PCR and the secretion of dopamine (DA) was assayed by ELISA. Then, 2 × 10 cells of SAF-treated NSC was administrated into PD rat models induced by 6-OHDA. The differentiation and survival of dopaminergic neurons was identified by fluorescence microscope and TH cells by immunostaining and DA secretion by ELISA at week 2 and week 4, respectively.
RESULTS
After being treated with SAF at 20 and 100 ng/mL for 1 week, TH and DAT positive rates increased 1.4- and 1.7-fold (p < 0.01, respectively). TH and DAT mRNA also increased 8.05- and 4.41-fold, respectively. And the release of DA statistically increased 1.5-fold (p < 0.01). In vivo, the number of rotations decreased to 4.33 ± 0.97 rpm (p < 0.01) and the survival rates increased to 77.66 ± 7.87% (p < 0.05) at week 4 after transplantation of SAF-treated NSC. Moreover, the transplanted cells increased three-fold, TH fluorescence density increased four-fold and DA releases increased 1.4-fold (p < 0.01) at week 4 after transplantation.
CONCLUSIONS
SAF promoted the production of functional DA cells and alleviated PD, which may contribute to a new therapy for PD patients.
背景
藏红花醛（SAF）已被证实具有促进神经生长的潜力。
目的
本研究验证 SAF 促进体外和体内多巴胺能神经元生长的作用。
材料和方法
用 1、20 或 100ng/ml 的 SAF 处理大鼠神经干细胞（NSC），通过流式细胞术和实时 PCR 检测酪氨酸羟化酶（TH）和多巴胺转运体（DAT）的表达水平，通过 ELISA 检测多巴胺（DA）的分泌。然后，将 SAF 处理的 NSC 2×10 个细胞注入由 6-OHDA 诱导的 PD 大鼠模型中。在第 2 周和第 4 周，通过荧光显微镜观察多巴胺能神经元的分化和存活，通过免疫染色观察 TH 细胞，通过 ELISA 观察 DA 分泌。
结果
用 20 和 100ng/ml 的 SAF 处理 1 周后，TH 和 DAT 阳性率分别增加了 1.4 倍和 1.7 倍（p<0.01）。TH 和 DAT mRNA 也分别增加了 8.05 倍和 4.41 倍。DA 的释放也统计学上增加了 1.5 倍（p<0.01）。体内，移植 SAF 处理的 NSC 后第 4 周，旋转次数减少至 4.33±0.97rpm（p<0.01），存活率增加至 77.66±7.87%（p<0.05）。此外，移植后第 4 周，移植细胞增加了 3 倍，TH 荧光密度增加了 4 倍，DA 释放增加了 1.4 倍（p<0.01）。
结论
SAF 促进了功能性 DA 细胞的产生，并缓解了 PD，这可能为 PD 患者提供一种新的治疗方法。
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