Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Beijing, China.
Insect Sci. 2014 Jun;21(3):278-90. doi: 10.1111/1744-7917.12112. Epub 2014 Mar 20.
The mitochondrial genome of Cervaphis quercus has been sequenced and annotated. The entire genome of 15,272 bp encodes two ribosomal RNA genes (rrnL and rrnS), 22 transfer RNA (tRNA) genes, 13 protein-coding genes and a control region. The genome has the same gene order as that found in the inferred ancestral insect. Nucleotide composition is highly A+T biased. All protein-coding genes use standard mitochondrial initiation codons. Secondary structure models of the two ribosomal RNA genes of C. quercus are similar to those proposed for other insects. All tRNAs have the classic clover-leaf structure, except for the dihydrouridine (DHU) arm of trnS (AGN), which forms a simple loop. The presence of structural elements in the control region is also discussed, with an emphasis on the possible regulation of replication and/or transcription. Comparison with mitochondrial genomes of other aphid species shows their gene arrangements are conserved; however, the variety of repeat regions in species from a different aphid subfamily, Aphidinae, suggests that they resulted from independent evolutionary events.
已经对栎肩腔蚧的线粒体基因组进行了测序和注释。整个基因组长 15272bp，编码两个核糖体 RNA 基因（rrnL 和 rrnS）、22 个转移 RNA（tRNA）基因、13 个蛋白编码基因和一个控制区。基因组具有与推断的昆虫祖先相同的基因排列顺序。核苷酸组成高度偏向 A+T。所有蛋白编码基因均使用标准的线粒体起始密码子。栎肩腔蚧的两个核糖体 RNA 基因的二级结构模型与其他昆虫提出的模型相似。除了 trnS（AGN）的二氢尿嘧啶（DHU）臂外，所有 tRNA 都具有典型的三叶草结构，该臂形成简单的环。还讨论了控制区中结构元件的存在，重点是复制和/或转录的可能调节。与其他蚜虫物种的线粒体基因组进行比较表明，它们的基因排列是保守的；然而，来自不同蚜虫亚科的物种中重复区域的多样性表明它们是独立进化事件的结果。
