Liang Yanxin, Du Zhenyong, Song Fan, He Jia
Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management College of Pant Protection, China Agricultural University, Beijing, China.
Institute of Plant Protection, Academy of Ningxia Agriculture and Forestry Science, Yinchuan, China.
Mitochondrial DNA B Resour. 2020 Nov 3;5(3):3685-3687. doi: 10.1080/23802359.2020.1832934.
This study completes the sequencing and annotation of the mitochondrial genome (mitogenome) of (Hemiptera: Aphididae) by using the high-throughput sequencing. The mitogenome is a typical circular DNA of 15,410 bp with 86.2% A + T content, and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, a repeat region between and , and a control region. The gene order follows the putative ancestral arrangements of insect mitogenome. All 13 protein-coding genes start with codon ATN and terminate with TAA or a single T. All tRNA genes have typical clover-leaf structure except for r. The control region is 638 bp in length with 86.0% A + T content. The phylogenetic tree supports the monophyly of Aphidini and Macrosiphini in Aphidinae and the sister relationship between and .
本研究利用高通量测序技术完成了（半翅目：蚜科）线粒体基因组（线粒体基因组）的测序和注释。线粒体基因组是一个典型的环状DNA，长度为15410bp，A+T含量为86.2%，由13个蛋白质编码基因、22个tRNA基因、2个rRNA基因、和之间的一个重复区域以及一个控制区域组成。基因顺序遵循昆虫线粒体基因组的假定祖先排列。所有13个蛋白质编码基因均以密码子ATN起始，以TAA或单个T终止。除r外，所有tRNA基因均具有典型的三叶草结构。控制区域长度为638bp，A+T含量为86.0%。系统发育树支持蚜亚科蚜族和长管蚜族的单系性以及和之间的姐妹关系。
