Department of Functional Anatomy, University School of Physical Education, Poznan, Poland.
Department of Biotechnology and Molecular Biology, Institute of Natural Fibres and Medicinal Plants, Poznan, Poland.
Arch Med Sci. 2013 Dec 30;9(6):1102-6. doi: 10.5114/aoms.2013.39212. Epub 2013 Nov 29.
The aim of this paper was to report the occurrence of peripheral blood chimerism in newborns from bigeminal pregnancies.
Cord blood collected from 50 pairs of twins constituted the biological material studied. Analyses included: DNA isolation, quantitative and qualitative assessment of DNA preparations, hybridization analysis of SLS type as well as of MLS type, and analysis of microsatellite sequences with regard to polymorphisms using polymerase chain reaction.
The presence of additional fragments of DNA in peripheral blood lymphocytes was found in four out of fifty pairs of monozygotic twins (8%) at locus D7S21 (7p22, n = 3) and locus D12S11 (12q24.3, n = 1). In these cases, the presence of additional DNA fragments was also proved by analysis of microsatellite sequence polymorphisms at loci HUMPLA2A1 (pancreatic phospholipase A-2, 12q23), HUMCYARO (cytochrome P450, 15q21.1) and HUMvWF (von Willebrand factor, 12p13).
The results of our study confirm the occurrence of chimerism in twins and constitutes the starting point for further studies aimed at determining the clinical significance of chimerism in twins both for women and fetuses.
本文旨在报告在双胎妊娠新生儿外周血嵌合体的发生情况。
收集了 50 对双胞胎的脐带血作为研究生物材料。分析包括：DNA 分离、DNA 制剂的定量和定性评估、SLS 型和 MLS 型杂交分析，以及使用聚合酶链反应对微卫星序列进行多态性分析。
在 4 对（8%）同卵双胞胎（n = 3）的外周血淋巴细胞中发现了 D7S21（7p22）和 D12S11（12q24.3）位点存在额外的 DNA 片段，在 1 对（2%）异卵双胞胎中发现了 D12S11（12q24.3）位点存在额外的 DNA 片段。在这些情况下，通过对位于 12q23 的 HUMPLA2A1（胰腺磷脂酶 A-2）、15q21.1 的 HUMCYARO（细胞色素 P450）和 12p13 的 HUMvWF（血管性血友病因子）的微卫星序列多态性分析也证明了额外 DNA 片段的存在。
我们的研究结果证实了双胞胎中嵌合体的发生，并为进一步研究嵌合体在双胞胎中的临床意义奠定了基础，包括对妇女和胎儿的影响。
Zotero 插件