Eufinger H, Rand S P, Schütte U
University Hospital of Oral & Maxillofacial Surgery-Regional Plastic Surgery, Knappschafts Hospital, Ruhr-University, Bochum, Germany.
Acta Genet Med Gemellol (Roma). 1995;44(1):25-30. doi: 10.1017/s0001566000001860.
Precision of zygosity determination in twins can be improved by the use of modern methods of DNA analysis. The clinical application of 4 single- (SLS) and 2 multi-locus (MLS), and 6 PCR (polymerase chain reaction) systems for zygosity determination in 12 twin pairs with oral clefts was compared with regard to the quality and quantity of sample material required and the probability of error in monozygosity determination. PCR systems proved to be superior to SLS or MLS, as DNA sampling is much more convenient, while its level of accuracy still fulfils clinical requirements. For this reason, PCR systems should be considered a basic method in modern clinical twin research.
通过使用现代DNA分析方法，可以提高双胞胎合子性判定的准确性。比较了4种单基因座（SLS）、2种多基因座（MLS）和6种聚合酶链反应（PCR）系统在12对患有腭裂的双胞胎中进行合子性判定时所需样本材料的质量和数量以及单合子性判定中的误差概率。事实证明，PCR系统优于SLS或MLS，因为DNA采样更加方便，同时其准确度仍能满足临床要求。因此，PCR系统应被视为现代临床双胞胎研究中的一种基本方法。
