一种用于检测甲胎蛋白变体的灵敏印迹系统，采用单克隆抗体和多克隆抗体。

A sensitive blotting system for detection of alpha-fetoprotein variants with monoclonal and polyclonal antibodies.

作者信息

Sittenfeld A, Moreno E

机构信息

Centro de Investigacion en Biologia Celular y Molecular, Universidad de Costa Rica, San Jose.

出版信息

J Immunol Methods. 1988 Jan 21;106(1):19-26. doi: 10.1016/0022-1759(88)90266-9.

DOI:10.1016/0022-1759(88)90266-9

PMID:2448384

Abstract

Human alpha-fetoprotein (AFP) variants from cord sera were separated by isoelectric focusing in agarose gels under native conditions, transferred to nitrocellulose paper and detected with polyclonal and monoclonal antibodies (Moabs). Rabbit anti-AFP recognized up to 9 individual electrophoretic variants in the range of pH 4.5 to pH 5.2. The reactivity of 8 Moabs ranged from weak to strong and showed variability in the pattern of AFP bands recognized. Moabs were separated into 3 groups according to the number of bands detected: group 1 detected 6 to 7 bands; group 2 recognized only one band; and group 3 recognized 4 bands. The sensitivity of the system with polyclonal antibodies was 0.15 ng of AFP in complete cord serum and varied between 300 and 0.2 ng with Moabs.

摘要

在天然条件下，通过琼脂糖凝胶等电聚焦法分离来自脐血血清的人甲胎蛋白（AFP）变体，将其转移至硝酸纤维素纸上，并用多克隆抗体和单克隆抗体（Moabs）进行检测。兔抗AFP可识别pH 4.5至pH 5.2范围内多达9种个体电泳变体。8种Moabs的反应性从弱到强不等，并且在识别的AFP条带模式上表现出变异性。根据检测到的条带数量，Moabs被分为3组：第1组检测到6至7条带；第2组仅识别1条带；第3组识别4条带。使用多克隆抗体的系统灵敏度在完整脐血血清中为0.15 ng AFP，使用Moabs时在300至0.2 ng之间变化。