Stephan Sarah, Guerra Diogo, Pospischil Andreas, Hilbe Monika, Weissenböck Herbert, Novotný Ladislav, Greub Gilbert, Croxatto Antony, Teifke Jens Peter, Ulrich Rainer G, Schlegel Mathias, Ruhl Silke, Schotte Ulrich, Binder Alfred, Sauer Sabine, Borel Nicole
1 Institute of Veterinary Pathology, University of Zurich, Vetsuisse Faculty Zurich, Winterthurerstrasse 268, 8057 Zurich, Switzerland.
J Wildl Dis. 2014 Apr;50(2):195-204. doi: 10.7589/2013-08-194. Epub 2014 Jan 31.
Few data are available on the occurrence of chlamydial infections in wild small mammals. We investigated the significance of free-living small mammals as reservoirs or transmission hosts for microorganisms of the phylum/class Chlamydiae. We obtained 3,664 tissue samples from 911 animals in Switzerland, Germany, Austria, the Czech Republic, and Afghanistan. Samples included internal organs (n = 3,652) and feces (n = 12) from 679 rodents (order Rodentia) and 232 insectivores (order Soricomorpha) and were tested by three TaqMan® real-time PCRs specific for members of the family Chlamydiaceae and selected Chlamydia-like organisms such as Parachlamydia spp. and Waddlia spp. Only one of 911 (0.11%) animals exhibited a questionable positive result by Chlamydiaceae-specific real-time PCR. Five of 911 animals were positive by specific real-time PCR for Parachlamydia spp. but could not be confirmed by quantitative PCR targeting the Parachlamydia acanthamoebae secY gene (secY qPCR). One of 746 animals (0.13%) was positive by real-time PCR for Waddlia chondrophila. This result was confirmed by Waddlia secY qPCR. This is the first detection of Chlamydia-like organisms in small wildlife in Switzerland. Considering previous negative results for Chlamydiaceae in wild ruminant species from Switzerland, these data suggest that wild small mammals are unlikely to be important carriers or transport hosts for Chamydiaceae and Chlamydia-like organisms.
关于野生小型哺乳动物中衣原体感染的发生情况，现有数据很少。我们调查了自由生活的小型哺乳动物作为衣原体门/纲微生物的储存宿主或传播宿主的重要性。我们从瑞士、德国、奥地利、捷克共和国和阿富汗的911只动物身上获取了3664份组织样本。样本包括679只啮齿动物（啮齿目）和232只食虫动物（鼩形目）的内部器官（n = 3652）和粪便（n = 12），并通过三种针对衣原体科成员和选定的衣原体样生物（如副衣原体属和瓦德利亚属）的TaqMan®实时荧光定量PCR进行检测。在911只动物中，只有1只（0.11%）通过衣原体科特异性实时荧光定量PCR呈现可疑阳性结果。911只动物中有5只通过副衣原体属特异性实时荧光定量PCR呈阳性，但无法通过靶向棘阿米巴副衣原体secY基因的定量PCR（secY qPCR）得到确认。746只动物中有1只（0.13%）通过嗜肺瓦德利亚菌实时荧光定量PCR呈阳性。这一结果通过瓦德利亚secY qPCR得到了确认。这是在瑞士小型野生动物中首次检测到衣原体样生物。考虑到之前瑞士野生反刍动物物种中衣原体科检测结果为阴性，这些数据表明野生小型哺乳动物不太可能是衣原体科和衣原体样生物的重要携带者或传播宿主。
