熊果酸通过激活PI3K/Akt信号通路诱导U937细胞分化。

Ursolic acid induces U937 cells differentiation by PI3K/Akt pathway activation.

作者信息

Deng Lin, Zhang Rui, Tang Feng, Li Chen, Xing Ying-Ying, Xi Tao

机构信息

School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China; Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing 210009, China; School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.

出版信息

Chin J Nat Med. 2014 Jan;12(1):15-9. doi: 10.1016/S1875-5364(14)60003-0.

DOI:10.1016/S1875-5364(14)60003-0

PMID:24484591

Abstract

AIM

Ursolic acid (UA), a pentacyclic triterpenoid, is used as an anti-inflammatory and anti-cancer agent. There were few studies on the effects of UA on differentiation, and this is the first time to elucidate the potential effect and molecular mechanism of UA on inducing differentiation in the human leukemia cell line U937.

METHODS

Wright-Giemsa staining, nitroblue tetrazolium reduction assay and flow cytometric analysis were utilized to demonstrate the differentiation of U937 cells induced by UA. Western blotting and immunofluorescence assay were used to investigate the possible mechanism.

RESULTS

It was found that UA could induce the differentiation of U937cells and Akt-activity was significantly increased during differentiation. Additionally, LY294002, a PI3K-Akt inhibitor, could block the differentiation of U937 cells induced by UA.

CONCLUSION

UA could induce the differentiation of U937 cells by activating the PI3K/Akt pathway, and it could be a potential candidate as a differentiation-inducing agent for the therapy of leukemia.

摘要