Dermatology Unit, Department of Medicine, University of Padua, Via Battisti 206, 35121 Padua, Italy.
Dermatology Unit, Department of Medicine, University of Padua, Via Battisti 206, 35121 Padua, Italy.
Acta Histochem. 2014 Jun;116(5):713-6. doi: 10.1016/j.acthis.2013.12.012. Epub 2014 Jan 30.
Pemphigus vulgaris is an autoimmune intraepithelial blistering skin disease characterized by the presence of circulating autoantibodies directed against surfaces of keratinocytes. Diagnosis is generally based on clinical features, histology, direct and indirect immunofluorescence and ELISA. This study describes a new BIOCHIP mosaic-based indirect immunofluorescence technique based on recombinant antigenic substrates and transfected cells. We investigated the diagnostic use of BIOCHIP for the serological diagnosis of Pemphigus vulgaris. Autoantibodies against desmoglein 3 were detected in 97.62% of patients (41/42) with P. vulgaris. There were no positive results in the negative control group. Our study revealed that BIOCHIP has high sensitivity and specificity comparable to that of the ELISA assays. Therefore the BIOCHIP technique seems to be an appropriate method for the diagnosis of P. vulgaris as it has been shown to be a simple, standardized and readily available novel tool, which could facilitate the diagnosis of this autoimmune bullous disease. We suggest that it could be used as an initial screening test to identify patients with P. vulgaris before using the ELISA approach.
寻常型天疱疮是一种自身免疫性表皮内水疱性皮肤病，其特征是存在针对角质形成细胞表面的循环自身抗体。诊断通常基于临床特征、组织学、直接和间接免疫荧光以及 ELISA。本研究描述了一种基于重组抗原底物和转染细胞的新型 BIOCHIP 镶嵌式间接免疫荧光技术。我们研究了 BIOCHIP 在寻常型天疱疮的血清学诊断中的应用。在 42 例寻常型天疱疮患者中，有 97.62%（41/42）检测到抗桥粒芯糖蛋白 3 的自身抗体。阴性对照组均无阳性结果。我们的研究表明，BIOCHIP 具有与 ELISA 检测相当的高灵敏度和特异性。因此，BIOCHIP 技术似乎是一种合适的寻常型天疱疮诊断方法，因为它已被证明是一种简单、标准化且易于获取的新型工具，可以促进这种自身免疫性大疱病的诊断。我们建议在使用 ELISA 方法之前，可以将其作为识别寻常型天疱疮患者的初始筛选测试。
