Department of Dermatology, The Royal Melbourne Hospital, Parkville, Victoria, Australia.
Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, Parkville, Victoria, Australia.
Australas J Dermatol. 2021 Aug;62(3):314-322. doi: 10.1111/ajd.13611. Epub 2021 May 26.
The BIOCHIP is an indirect immunofluorescence diagnostic investigation which identifies multiple autoantibodies with a mosaic panel of target antigen-specific substrates in a single incubation field. The EUROIMMUN Dermatology Profile ELISA allows simultaneous investigation of the six most important autoantibodies in bullous autoimmune dermatoses. Evaluation of the BIOCHIP Mosaic 7, compared to that of the EUROIMMUN Dermatology Profile ELISA, when used as a diagnostic investigation in pemphigus and pemphigoid, was undertaken in an Australian cohort.
The serum of 27 patients was analysed including patients with pemphigus vulgaris (n = 10), pemphigus foliaceous (n = 4), bullous pemphigoid (n = 8), mucous membrane pemphigoid (n = 3) and negative controls (n = 2). Results of the BIOCHIP were compared with the EUROIMMUN Dermatology Profile ELISA, as well as with histology, direct immunofluorescence and indirect immunofluorescence.
In pemphigus vulgaris, sensitivity & specificity for the BIOCHIP Mosaic 7 were 100% and 94.1%, comparable to that of the EUROIMMUN Dermatology Profile ELISA with 80% sensitivity and 100% specificity. In bullous pemphigoid, sensitivity of the BIOCHIP was 87.5% and sensitivity of the EUROIMMUN Dermatology ELISA profile was 75%, whilst specificities for both diagnostic methods were 100% in our limited cohort. There was substantial or almost perfect concordance between the BIOCHIP Mosaic 7 and EUROIMMUN Dermatology Profile ELISA for pemphigus vulgaris and bullous pemphigoid.
The BIOCHIP Mosaic 7 is a rapid, reliable diagnostic investigation in pemphigus and bullous pemphigoid. Results indicate it is comparable to the EUROIMMUN Dermatology Profile ELISA, whilst also providing additional testing with salt split skin, on one field.
BIOCHIP 是一种间接免疫荧光诊断检测方法，可在单个孵育场中使用针对多种靶抗原特异性底物的马赛克面板来识别多种自身抗体。EUROIMMUN 皮肤病学分析 ELISA 允许同时检测六种在大疱性自身免疫性皮肤病中最重要的自身抗体。在澳大利亚队列中，对 BIOCHIP Mosaic 7 进行了评估，与 EUROIMMUN 皮肤病学分析 ELISA 相比，当用于天疱疮和类天疱疮的诊断研究时。
分析了 27 例患者的血清，包括寻常型天疱疮（n=10）、落叶型天疱疮（n=4）、大疱性类天疱疮（n=8）、黏膜类天疱疮（n=3）和阴性对照（n=2）。比较了 BIOCHIP 的结果与 EUROIMMUN 皮肤病学分析 ELISA，以及组织学、直接免疫荧光和间接免疫荧光。
在寻常型天疱疮中，BIOCHIP Mosaic 7 的敏感性和特异性分别为 100%和 94.1%，与 EUROIMMUN 皮肤病学分析 ELISA 的 80%敏感性和 100%特异性相当。在大疱性类天疱疮中，BIOCHIP 的敏感性为 87.5%，EUROIMMUN 皮肤病学分析 ELISA 谱的敏感性为 75%，而在我们有限的队列中，两种诊断方法的特异性均为 100%。BIOCHIP Mosaic 7 与 EUROIMMUN 皮肤病学分析 ELISA 在寻常型天疱疮和大疱性类天疱疮之间存在实质性或几乎完全一致。
BIOCHIP Mosaic 7 是天疱疮和大疱性类天疱疮的快速、可靠的诊断检测方法。结果表明，它与 EUROIMMUN 皮肤病学分析 ELISA 相当，同时还在一个场地上提供了盐分裂皮肤的额外检测。
