Roncalli M, Sideri M, Giè P, Servida E
Department of Pathology, Hospital of Milan, Italy.
Lab Invest. 1988 Feb;58(2):141-9.
The immunocompetent cell population of the cervical transformation zone of 18 uteri removed for noncervical disease, has been investigated with monoclonal antibodies. The panel included Leu 2a, 3a, 4, 14, and IL II receptor for lymphocytes and T cell subsets, Leu 7 for NK cells, Leu M5, Leu 10, HLA-DR, DRC 1 for dendritic cells, and Leu 6 for Langerhans' cells (LC). In ectocervical epithelium HLA-DR, Leu 6 and Leu 10 antibodies identified subpopulations of dendritic cells which differed in number and in topographic distribution. Furthermore, a strong HLA-DR epithelial positivity was constantly observed in endocervical columnar cells as well as in keratinocytes of squamous metaplasia. Leu 2a+ cells (T suppressor/cytotoxic) prevailed in the stromal and epithelial compartments of ecto/endocervix; in 6 cases, however, Leu 3a+ cells (T helper/inducer) represented the main T cell subset in the ectocervical stroma. B lymphocytes were occasionally noticed in the subepithelial stroma while NK and DRC-1 cells were never observed. Finally, only few lymphocytes displayed a positivity for IL II receptor. This study suggests that several phenotypes of intraepithelial dendritic cells are present in the transformation zone and that endocervical columnar cells and keratinocytes of squamous metaplasia express HLA-DR products; the latter finding may be related to the presence of intraepithelial and stromal T lymphocytes.
对18例因非宫颈疾病而切除的子宫宫颈转化区的免疫活性细胞群，采用单克隆抗体进行了研究。该抗体组合包括用于淋巴细胞和T细胞亚群的Leu 2a、3a、4、14和IL II受体，用于NK细胞的Leu 7，用于树突状细胞的Leu M5、Leu 10、HLA-DR、DRC 1，以及用于朗格汉斯细胞（LC）的Leu 6。在外宫颈上皮中，HLA-DR、Leu 6和Leu 10抗体识别出数量和地形分布不同的树突状细胞亚群。此外，在内宫颈柱状细胞以及鳞状化生的角质形成细胞中，始终观察到强烈的HLA-DR上皮阳性。Leu 2a+细胞（T抑制/细胞毒性）在外/内宫颈的基质和上皮区占优势；然而，在6例中，Leu 3a+细胞（T辅助/诱导）是外宫颈基质中的主要T细胞亚群。B淋巴细胞偶尔出现在上皮下基质中，而从未观察到NK和DRC-1细胞。最后，只有少数淋巴细胞对IL II受体呈阳性。这项研究表明，转化区存在几种上皮内树突状细胞表型，并且内宫颈柱状细胞和鳞状化生的角质形成细胞表达HLA-DR产物；后一发现可能与上皮内和基质T淋巴细胞的存在有关。
