Concordia University, Montréal, QC H4B 1R6, Canada.
Concordia University, Montréal, QC H4B 1R6, Canada.
Psychoneuroendocrinology. 2014 Feb;40:69-75. doi: 10.1016/j.psyneuen.2013.10.002. Epub 2013 Oct 16.
Compliance with awakening salivary sampling is important for precise measurement of the diurnal cortisol profile. During childhood and adolescence, developmental factors influence sampling upon awakening (awake0) due to school routine, sleep/wake patterns, and age related cortisol changes. In the present study, children and adolescents' sampling compliance of awakening cortisol was evaluated using accelerometry. Children and adolescents (N=201; 45.3% female; 8-18 years; Mage=12.68 years, SD=2.03) participating in the Healthy Heart Project collected saliva samples, wore a tri-axle accelerometer, and completed demographic questionnaires. Intra-class correlations derived to examine awake0 sampling compliance indicated children and adolescents were highly compliant (ICC=.98). In children, a delay in awake0 sampling was associated with a steeper diurnal slope (β=-.23, p=.037) and greater awake0 cortisol (β=.24, p=.024); this was not observed in adolescents. In summary, children and adolescents are compliant with awakening salivary sampling. Sampling delay, particularly in children, and time of awakening influenced measures of the diurnal cortisol profile. These findings inform future studies assessing the diurnal cortisol profile in children and adolescents.
遵守觉醒唾液采样对于精确测量日间皮质醇谱非常重要。在儿童和青少年时期，由于学校常规、睡眠/觉醒模式和与年龄相关的皮质醇变化，发育因素会影响觉醒时（觉醒 0 时）的采样。在本研究中，使用加速度计评估了儿童和青少年觉醒皮质醇采样的依从性。参加“健康心脏计划”的儿童和青少年（N=201；女性占 45.3%；8-18 岁；平均年龄=12.68 岁，标准差=2.03）采集唾液样本、佩戴三轴加速度计并完成人口统计学问卷。为检查觉醒 0 采样依从性而得出的组内相关系数表明，儿童和青少年的依从性很高（ICC=.98）。在儿童中，觉醒 0 采样延迟与更陡峭的日间斜率（β=-.23，p=.037）和更高的觉醒 0 皮质醇（β=.24，p=.024）相关；而在青少年中则没有观察到这种情况。总之，儿童和青少年对觉醒唾液采样具有较高的依从性。采样延迟，尤其是在儿童中，以及觉醒时间会影响日间皮质醇谱的测量。这些发现为未来评估儿童和青少年日间皮质醇谱的研究提供了信息。
