Leibniz Centre for Working Environment and Human Factors at TU Dortmund (Leibniz-Institut für Arbeitsforschung an der TU Dortmund), Germany.
Int J Psychophysiol. 2011 Nov;82(2):202-5. doi: 10.1016/j.ijpsycho.2011.08.005. Epub 2011 Aug 30.
Cortisol awakening responses (CAR) are often blunted or even extinguished when saliva samplings are delayed. The literature suggests that delays of 10 or 15 min are tolerable. Delays of 15 min were analysed with 510 CARs with the first sample taken within 3 min post-awakening followed by 4 samples taken 15, 30, 45, and 60 min later. Varying delays of up to 30 min were analysed with these and further 148 CARs where sampling began within 30 min post-awakening. Times of awakening and of saliva collection were verified by polysomnography or actimetry and by electronic devices respectively. Simulated sampling delays of 15 min revealed highly significant deviations from correctly taken CARs. No deviations were found for delays of up to 11.5 min suggesting that delays of up to 10 min are acceptable but that delays between 10 and 15 min might become critical.
皮质醇觉醒反应（CAR）在唾液采样延迟时常常减弱甚至消失。文献表明，延迟 10 或 15 分钟是可以接受的。分析了 510 次 CAR，其中第一次样本在觉醒后 3 分钟内采集，然后在 15、30、45 和 60 分钟后采集 4 个样本。进一步分析了这些样本和另外 148 个 CAR，这些样本的采集是在觉醒后 30 分钟内开始的，最大延迟可达 30 分钟。觉醒时间和唾液采集时间分别通过多导睡眠图或活动计和电子设备进行验证。模拟的 15 分钟采样延迟显示出与正确采集的 CAR 存在高度显著的偏差。对于延迟不超过 11.5 分钟的情况，未发现偏差，这表明延迟不超过 10 分钟是可以接受的，但 10 到 15 分钟之间的延迟可能会变得关键。
