携带牛白血病病毒两种X基因产物抗原决定簇的β-半乳糖苷酶融合蛋白在细菌中的表达。
Expression in bacteria of beta-galactosidase fusion proteins carrying antigenic determinants of the two X gene products of bovine leukemia virus.
作者信息
Willems L, Chen G, Burny A, Kettmann R
机构信息
Faculty of Agronomy, Gembloux, Belgium.
出版信息
Leukemia. 1988 Jan;2(1):1-5.
PMID:2448554
Abstract
A cDNA corresponding to the bovine leukemia virus post-envelope region (X gene) was subcloned into the lambda gt11 expression vector. Two large protein fragments corresponding respectively to the long and short open reading frames of the X region were expressed as beta-galactosidase fusion proteins. These products were specifically recognized by sera from bovine leukemia virus-infected cattle.
摘要
将一段与牛白血病病毒包膜后区域（X基因）对应的cDNA亚克隆到λgt11表达载体中。分别对应于X区域长开放阅读框和短开放阅读框的两个大蛋白片段被表达为β-半乳糖苷酶融合蛋白。这些产物被来自感染牛白血病病毒的牛的血清特异性识别。
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