Yoshinaka Y, Oroszlan S
Biochem Biophys Res Commun. 1985 Aug 30;131(1):347-54. doi: 10.1016/0006-291x(85)91809-1.
Partial sequence analysis of a 14 kilodalton protein (p14), synthesized by in vitro translation of bovine leukemia virus genomic RNA, showed that it is encoded in the 'X' region of proviral DNA, located between the env gene and the 3' long terminal repeat. The 'X' gene contains a short and a long open reading frame (X-SORF and X-LORF) which overlap. BLV p14x is specified by X-SORF and not X-LORF as seen with the related human T-cell leukemia virus which expresses p38-40x. Antibodies in sera from animals with BLV induced tumors were shown to recognize p14x. Expression of this protein in natural infection might be important for virus replication and/or for BLV induced oncogenesis.
通过对牛白血病病毒基因组RNA进行体外翻译合成的一种14千道尔顿蛋白（p14）进行部分序列分析，结果表明它由原病毒DNA的“X”区域编码，该区域位于env基因和3'长末端重复序列之间。“X”基因包含一个短开放阅读框和一个长开放阅读框（X-SORF和X-LORF），二者相互重叠。与表达p38-40x的相关人类T细胞白血病病毒不同，BLV p14x由X-SORF而非X-LORF指定。研究表明，感染BLV并诱发肿瘤的动物血清中的抗体可识别p14x。这种蛋白在自然感染中的表达可能对病毒复制和/或BLV诱导的肿瘤发生具有重要意义。
