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线粒体RNA剪接缺陷的核抑制：MRS3基因的核苷酸序列及破坏

Nuclear suppression of a mitochondrial RNA splice defect: nucleotide sequence and disruption of the MRS3 gene.

作者信息

Schmidt C, Söllner T, Schweyen R J

机构信息

Institut für Genetik und Mikrobiologie, Universität München, Federal Republic of Germany.

出版信息

Mol Gen Genet. 1987 Nov;210(1):145-52. doi: 10.1007/BF00337771.

DOI:10.1007/BF00337771
PMID:2448588
Abstract

A mitochondrial RNA splice defect in the first intron of the COB gene (bI1) can be suppressed by a dominant nuclear mutation SUP-101. Starting with a gene bank of yeast nuclear DNA from a SUP-101 suppressor strain cloned in the YEp13 plasmid, we have isolated a recombinant plasmid which exerts a suppressor activity similar to the SUP-101 allele. The N3(2) insert of this plasmid contains an open reading frame (ORF) of 1014 bp which is transcribed to a 12 S RNA. Deletion of the 5' end of this ORF and its upstream sequences abolishes the suppressor activity. The N3(2) insert thus carries a functional gene (called MRS3) which can suppress a mitochondrial splice defect. The chromosomal equivalent of the cloned gene has been mapped to chromosome 10. Disruption of this chromosomal gene has no phenotypic effect on wild-type cells.

摘要

细胞色素b基因（bI1）第一个内含子中的线粒体RNA剪接缺陷可被显性核突变SUP - 101抑制。从克隆于YEp13质粒的SUP - 101抑制菌株的酵母核DNA基因库开始，我们分离出了一种重组质粒，其发挥的抑制活性与SUP - 101等位基因相似。该质粒的N3(2)插入片段包含一个1014 bp的开放阅读框（ORF），转录为12 S RNA。该ORF 5'端及其上游序列的缺失消除了抑制活性。因此，N3(2)插入片段携带一个功能性基因（称为MRS3），其可抑制线粒体剪接缺陷。克隆基因的染色体对应物已定位到第10号染色体。该染色体基因的破坏对野生型细胞没有表型影响。

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本文引用的文献

1
Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron.细胞色素b野生型和box3突变体中内含子及侧翼外显子的序列揭示了一个由内含子编码的交错剪接蛋白。
Cell. 1980 Nov;22(2 Pt 2):333-48. doi: 10.1016/0092-8674(80)90344-x.
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Long range control circuits within mitochondria and between nucleus and mitochondria. I. Methodology and phenomenology of suppressors.线粒体内以及细胞核与线粒体之间的远程控制电路。I. 抑制因子的方法学与现象学
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Determination of functional domains in intron bI1 of yeast mitochondrial RNA by studies of mitochondrial mutations and a nuclear suppressor.
酵母中众多II组内含子剪接缺陷的抑制因子。
Curr Genet. 1993 Oct;24(4):301-6. doi: 10.1007/BF00336780.
4
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Mol Gen Genet. 1989 Feb;215(3):517-28. doi: 10.1007/BF00427051.
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Proc Natl Acad Sci U S A. 1989 Apr;86(7):2286-90. doi: 10.1073/pnas.86.7.2286.
6
Three nuclear genes suppress a yeast mitochondrial splice defect when present in high copy number.当以高拷贝数存在时，三个核基因可抑制酵母线粒体剪接缺陷。
Curr Genet. 1987;12(7):503-9. doi: 10.1007/BF00419559.
7
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8
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