Balzi E, Chen W N, Capieaux E, McCusker J H, Haber J E, Goffeau A
Unité de Biochimie Physiologique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
Gene. 1989 Nov 30;83(2):271-9. doi: 10.1016/0378-1119(89)90113-3.
In Saccharomyces cerevisiae, the SCL-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCL1-1 crl3-2 strain. The scl1+ mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from scl1+ possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of scl1+ demonstrates that it is an essential gene.
在酿酒酵母中，SCL-1突变是抗环己酰亚胺、温度敏感（ts）致死突变crl3的显性抑制因子[麦卡斯基尔和哈伯，《遗传学》119（1988a）303 - 315]。通过筛选TRP5和LEU1之间35 kb区域的亚克隆，以恢复SCL1-1 crl3-2菌株的ts表型，从而分离出野生型scl1+基因。scl1+ mRNA约900 nt长，编码一个810 bp的开放阅读框。从scl1+推导的多肽具有一个假定的分泌信号肽。5'-非编码区可能受多种调控，因为它与DNA结合蛋白GCN4、GFI以及可能的TUF的共有序列具有显著同源性。scl1+的基因破坏表明它是一个必需基因。
