van Eden W, Thole J E, van der Zee R, Noordzij A, van Embden J D, Hensen E J, Cohen I R
Department of Infectious Diseases and Immunology, Veterinary Faculty, State University of Utrecht, The Netherlands.
Nature. 1988 Jan 14;331(6152):171-3. doi: 10.1038/331171a0.
Adjuvant arthritis (AA) is a chronic disease inducible in rats by immunization with an antigen of Mycobacterium tuberculosis. After the isolation of arthritogenic T-cell lines and clones, it became possible to demonstrate that the critical M. tuberculosis antigen contained an epitope cross-reactive with a self-antigen in joint cartilage. Like AA rats, patients suffering from rheumatoid arthritis demonstrated specific T-lymphocyte reactivity to the M. tuberculosis fraction containing the cross-reactive epitope. To characterize the critical M. tuberculosis epitope we used AA T-cell clones to screen mycobacterial antigens expressed in Escherichia coli and genetically engineered truncated proteins and synthetic peptides. The AA T-cell clones recognized an epitope formed by the amino acids at positions 180-188 in the sequence of a Mycobacterium bovis BCG antigen. Administration of this antigen to rats induced resistance to subsequent attempts to produce AA.
佐剂性关节炎（AA）是一种通过用结核分枝杆菌抗原免疫大鼠诱导产生的慢性疾病。在分离出致关节炎T细胞系和克隆后，得以证明关键的结核分枝杆菌抗原含有一个与关节软骨中的自身抗原交叉反应的表位。与佐剂性关节炎大鼠一样，类风湿性关节炎患者对含有交叉反应表位的结核分枝杆菌组分表现出特异性T淋巴细胞反应性。为了表征关键的结核分枝杆菌表位，我们使用佐剂性关节炎T细胞克隆筛选在大肠杆菌中表达的分枝杆菌抗原、基因工程截短蛋白和合成肽。佐剂性关节炎T细胞克隆识别由牛分枝杆菌卡介苗抗原序列中第180 - 188位氨基酸形成的一个表位。给大鼠施用该抗原可诱导其对随后产生佐剂性关节炎的尝试产生抗性。
