Institute of Aging Research, Guangdong Medical College, Dongguan, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, China.
Institute of Aging Research, Guangdong Medical College, Dongguan, China; Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan, China; Institute of Biochemistry & Molecular Biology, Guangdong Medical College, Zhanjiang, China.
Mech Ageing Dev. 2014 Jun;138:53-8. doi: 10.1016/j.mad.2014.01.007. Epub 2014 Jan 31.
Saccharomyces cerevisiae Nar1p is an essential Fe/S protein that exhibits striking similarity to bacterial iron-only hydrogenases. Nar1p is required for the maturation of cytosolic and nuclear, but not of mitochondrial Fe/S proteins, and plays a role in modulating sensitivity to oxygen in both yeast and Caenorhabditis elegans through unknown mechanisms. Here we report that Nar1 deficiency results in shortened lifespan and sensitivity to paraquat that is rescued by increased expression of mitochondrial superoxide dismutase. These data suggest that Nar1p promotes protection against oxidative stress and define a new role for Nar1p in promoting replicative lifespan.
酿酒酵母 Nar1p 是一种必需的 Fe/S 蛋白，与细菌铁单加氧酶具有惊人的相似性。Nar1p 是细胞质和核内 Fe/S 蛋白成熟所必需的，但线粒体 Fe/S 蛋白的成熟则不需要它，而且通过未知的机制在酵母和秀丽隐杆线虫中发挥着调节对氧敏感性的作用。在这里，我们报告 Nar1 缺失导致寿命缩短和对百草枯的敏感性增加，而过表达线粒体超氧化物歧化酶可以挽救这种表型。这些数据表明 Nar1p 促进了对氧化应激的保护，并定义了 Nar1p 在促进复制寿命方面的新作用。
