GMFG 高表达与不良预后相关，并促进卵巢上皮性癌的细胞迁移和侵袭。
High GMFG expression correlates with poor prognosis and promotes cell migration and invasion in epithelial ovarian cancer.
机构信息
Women's Reproductive Health Laboratory of Zhejiang Province, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
出版信息
Gynecol Oncol. 2014 Mar;132(3):745-51. doi: 10.1016/j.ygyno.2014.01.044. Epub 2014 Jan 31.
OBJECTIVE
The aim of this study was to characterize the clinical significance of GMFG, a novel ADF/cofilin superfamily protein, and investigate its role in cell migration and invasion in epithelial ovarian cancer (EOC).
METHODS
The expression of GMFG in EOC tissues and ovarian cancer cell lines was evaluated by immunohistochemistry and immunoblotting respectively. The data were statistically analyzed for the associations of GMFG expression with clinicopathologic parameters and survival. In vitro cell migration and invasion assays were performed to determine the role of GMFG in cell migratory behaviors. The effect of GMFG on reorganization of actin cytoskeleton was investigated by immunostaining.
RESULTS
GMFG was overexpressed in EOC. Up-regulated GMFG expression was closely correlated with advanced FIGO stage and chemoresistance of the disease. EOC patients with higher GMFG expression showed poorer progression-free survival (PFS) and overall survival (OS). In vitro cellular assays revealed that GMFG promoted cell migration and invasion. GMFG expression altered actin cytoskeleton organization probably by interacting with the Arp2/3 complex.
CONCLUSION
GMFG expression independently predicts poorer prognosis in patients with EOC. Ectopic overexpression of GMFG contributes to the malignant biological behavior of ovarian cancer cells.
目的
本研究旨在探讨 GMFG（一种新型 ADF/cofilin 超家族蛋白）的临床意义，并研究其在卵巢上皮性癌（EOC）细胞迁移和侵袭中的作用。
方法
采用免疫组织化学和免疫印迹法分别评估 GMFG 在 EOC 组织和卵巢癌细胞系中的表达。对 GMFG 表达与临床病理参数和生存的相关性进行统计学分析。通过体外细胞迁移和侵袭实验来确定 GMFG 在细胞迁移行为中的作用。通过免疫染色研究 GMFG 对肌动蛋白细胞骨架重组的影响。
结果
GMFG 在 EOC 中过表达。上调的 GMFG 表达与 FIGO 晚期分期和疾病的化疗耐药密切相关。GMFG 表达较高的 EOC 患者无进展生存期（PFS）和总生存期（OS）更差。体外细胞实验表明，GMFG 促进了细胞迁移和侵袭。GMFG 表达通过与 Arp2/3 复合物相互作用改变肌动蛋白细胞骨架的组织。
结论
GMFG 的表达独立预测 EOC 患者预后不良。GMFG 的异位过表达有助于卵巢癌细胞的恶性生物学行为。
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