Silva Vanessa R, Marcondes P, Silva M, Villaverde Antonio B, Castro-Faria-Neto Hugo C, Vieira Rodolfo P, Aimbire Flavio, de Oliveira Ana Paula L
Nove de Julho University - UNINOVE, Laboratory of Pulmonary and Exercise Immunology - LABPEI, Rua Vergueiro 239/245, PO Box 01504-001, São Paulo, SP, Brazil.
Department of Science and Technology, Federal University of São Paulo, PO Box 12231-280, São José dos Campos, SP, Brazil.
Respir Physiol Neurobiol. 2014 Apr 1;194:37-48. doi: 10.1016/j.resp.2014.01.008. Epub 2014 Jan 28.
Low-level laser therapy (LLLT) controls bronchial hyperresponsiveness (BHR) associated with increased RhoA expression as well as pro-inflammatory mediators associated with NF-kB in acute lung inflammation. Herein, we explore if LLLT can reduce both BHR and Th2 cytokines in allergic asthma. Mice were studied for bronchial reactivity and lung inflammation after antigen challenge. BHR was measured through dose-response curves to acetylcholine. Some animals were pretreated with a RhoA inhibitor before the antigen. LLLT (660 nm, 30 mW and 5.4 J) was applied on the skin over the right upper bronchus and two irradiation protocols were used. Reduction of BHR post LLLT coincided with lower RhoA expression in bronchial muscle as well as reduction in eosinophils and eotaxin. LLLT also diminished ICAM expression and Th2 cytokines as well as signal transducer and activator of transduction 6 (STAT6) levels in lungs from challenged mice. Our results demonstrated that LLLT reduced BHR via RhoA and lessened allergic lung inflammation via STAT6.
低强度激光疗法（LLLT）可控制与急性肺炎症中RhoA表达增加相关的支气管高反应性（BHR）以及与NF-κB相关的促炎介质。在此，我们探讨LLLT是否能降低过敏性哮喘中的BHR和Th2细胞因子。在抗原激发后，对小鼠的支气管反应性和肺部炎症进行了研究。通过对乙酰胆碱的剂量反应曲线来测量BHR。一些动物在抗原刺激前用RhoA抑制剂进行预处理。将LLLT（660纳米，30毫瓦和5.4焦耳）应用于右上支气管上方的皮肤，并使用了两种照射方案。LLLT后BHR的降低与支气管肌肉中RhoA表达的降低以及嗜酸性粒细胞和嗜酸性粒细胞趋化因子的减少相一致。LLLT还减少了受攻击小鼠肺部的细胞间黏附分子（ICAM）表达、Th2细胞因子以及信号转导和转录激活因子6（STAT6）水平。我们的结果表明，LLLT通过RhoA降低BHR，并通过STAT6减轻过敏性肺部炎症。
