Yin Jun-Xiang, Tang Zhiwei, Gan Yan, Li Lejun, Shi Fudong, Coons Stephen, Shi Jiong
Department of Neurology, Barrow Neurological Institute, 350 W Thomas Road, Phoenix, AZ 85013, USA.
Department of Neuropathology, Barrow Neurological Institute, 350 W Thomas Road, Phoenix, AZ 85013, USA.
Neuropharmacology. 2014 Jun;81:1-5. doi: 10.1016/j.neuropharm.2014.01.027. Epub 2014 Jan 29.
Pertussis toxin (PTx) has various effects in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). This study was designed to explore the protective effects of PTx of different doses and subunits. EAE model was induced with myelin oligodendrocyte glycoprotein (MOG35-55, 200 ug) plus complete Freund's adjuvant in 6-7 week-old female C57BL/6 mice. PTx reduced clinical deficits of EAE by 91.3%. This reduction in clinical deficits was achieved by attenuating demyelination by 75.5%. Furthermore, PTx reduced the lymphocyte infiltration, deactivated microglia activation and changed T cell profile by increasing T helper (type 1 and 2) and T regulatory cells.
百日咳毒素（PTx）在实验性自身免疫性脑脊髓炎（EAE，一种多发性硬化症（MS）的动物模型）中具有多种作用。本研究旨在探讨不同剂量和亚基的PTx的保护作用。在6 - 7周龄雌性C57BL/6小鼠中，用髓鞘少突胶质细胞糖蛋白（MOG35 - 55，200μg）加完全弗氏佐剂诱导EAE模型。PTx使EAE的临床缺陷减少了91.3%。通过使脱髓鞘减轻75.5%实现了临床缺陷的这种减少。此外，PTx减少了淋巴细胞浸润，使小胶质细胞活化失活，并通过增加辅助性T细胞（1型和2型）和调节性T细胞改变了T细胞谱。
