分段负链 RNA 病毒核蛋白结构。

Segmented negative strand RNA virus nucleoprotein structure.

机构信息

European Molecular Biology Laboratory, Grenoble Outstation and UJF-EMBL-CNRS International Unit of Virus Host-Cell Interactions, 6 rue Jules Horowitz, BP181, Grenoble Cedex 9 38042, France.

Department of Microbiology and Molecular Medicine, University of Geneva School of Medicine, CMU, 1 rue Michel-Servet, Geneva 1211, Switzerland.

出版信息

Curr Opin Virol. 2014 Apr;5:7-15. doi: 10.1016/j.coviro.2014.01.003. Epub 2014 Jan 30.

DOI:10.1016/j.coviro.2014.01.003

PMID:24486721

Abstract

Negative strand RNA virus (NSV) genomes are never free, but always found assembled with multiple copies of their nucleoprotein, as RNPs. A flurry of papers describing the X-ray crystal structures of several segmented NSV nucleoproteins have recently appeared. The most significant feature of these various structures is that the arms that are used to oligomerize the nucleoproteins on their genome RNAs are highly flexible, permitting these RNPs to assume virtually unlimited geometries. The structural flexibility of segmented NSV RNPs is undoubtedly important in all aspects of their biology, including genome replication and circularization, and the selection of one copy of each segment for packaging into virus particles.

摘要

负链 RNA 病毒 (NSV) 基因组从不自由，但总是与多个核蛋白拷贝组装在一起，形成 RNP。最近出现了大量描述几种分段 NSV 核蛋白 X 射线晶体结构的论文。这些不同结构的最显著特征是，用于在其基因组 RNA 上寡聚化核蛋白的臂高度灵活，允许这些 RNP 采用几乎无限的几何形状。分段 NSV RNP 的结构灵活性无疑在其生物学的各个方面都很重要，包括基因组复制和环化，以及选择每个片段的一个拷贝包装到病毒颗粒中。