Hacettepe University, Faculty of Science, Department of Biology, Beytepe Campus, 06800, Ankara, Turkey.
Hacettepe University, Faculty of Science, Department of Biology, Beytepe Campus, 06800, Ankara, Turkey.
Toxicol Lett. 2014 Apr 7;226(1):35-42. doi: 10.1016/j.toxlet.2014.01.030. Epub 2014 Jan 30.
Chemicals that occur in vegetal food and known as phytoestrogens, because of their structures similarity to estrogen, have benefits on chronic diseases. Despite this, when they are taken at high amounts, they can cause harmful effects on endocrine system of human and animals. In this study, it has been intended to determine the estrogenic potencies of phytoestrogens apigenin, phloretin and myricetin whose affinities for estrogen receptors in vitro. The female rats divided into 17 groups, each containing six rats. There was a negative control group and there were positive control dose groups which contains ethinyl estradiol, ethinyl estradiol+tamoxifen and genistein. The other dose groups which were tested for estrogenic activity contains apigenin, myricetin and phloretin All chemicals have been given to Wistar immature female rats with oral gavage for 3 consecutive days. By using uterotrophic analysis, uterus wet and blotted weights, vaginal opening, uterus length of female rats has been recorded at the end of the experiment. For detect of cell response, luminal epithelium height, gland number and lactoferrin intensity in luminal epithelium of uterus were evaluated. Biochemical analysises in blood were performed. Relative uterus weights of rats in 100 mg/kg/day dose group of myricetin were statistically increased according to vehicle control and positive control groups. In dose groups of apigenin and phloretin it was found that there were cell responses in uterus. All treatment groups had a significant difference in the high intensity of lactoferrin and uterine gland count compared to oil control group. There was no difference between phloretin and apigenin treatment groups in uterine weight statictically. Uterine heights were increased in positive control groups and 100 mg/kg/day dose group of myricetin. Epithelial cell heights were increased in treatment groups except apigenin and phloretin dose groups. There was no difference between all treatment groups in vaginal opening values according to positive control.
植物性食物中存在的化学物质被称为植物雌激素，由于它们的结构与雌激素相似，对慢性疾病有一定益处。然而，当它们被大量摄入时，可能会对人类和动物的内分泌系统造成有害影响。在这项研究中，旨在确定植物雌激素芹菜素、根皮素和杨梅素的雌激素活性，体外研究它们与雌激素受体的亲和力。将雌性大鼠分为 17 组，每组 6 只。有一个阴性对照组和阳性对照组，阳性对照组含有炔雌醇、炔雌醇+他莫昔芬和染料木黄酮。其他测试雌激素活性的剂量组含有芹菜素、杨梅素和根皮素。所有化学物质均通过口服灌胃连续 3 天给予 Wistar 未成熟雌性大鼠。通过子宫增重分析、子宫湿重和干重、阴道开口、子宫长度来记录实验结束时雌性大鼠的情况。为了检测细胞反应，评估子宫腔上皮细胞的高度、腺数量和腔上皮细胞中的乳铁蛋白强度。对血液进行生化分析。与载体对照组和阳性对照组相比，杨梅素 100mg/kg/天剂量组的大鼠相对子宫重量呈统计学增加。在芹菜素和根皮素剂量组中，发现子宫有细胞反应。与油对照组相比，所有治疗组的乳铁蛋白高强度和子宫腺计数均有显著差异。芹菜素和根皮素治疗组的子宫重量无统计学差异。阳性对照组和杨梅素 100mg/kg/天剂量组的子宫高度增加。除了芹菜素和根皮素剂量组外，治疗组的上皮细胞高度均增加。根据阳性对照组，所有治疗组的阴道开口值无差异。
