College of Korean Medicine, Gachon University, Seongnam 13120, Korea.
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Molecules. 2021 Dec 22;27(1):36. doi: 10.3390/molecules27010036.
(Scop.) Sing., a mushroom of the family , has been used in traditional oriental medicine to treat cholecystitis, improve bile secretion, and regulate bile-duct pressure. The present study evaluated the estrogen-like effects of using a cell-proliferation assay in an estrogen-receptor-positive breast cancer cell line (MCF-7). We found that the methanol extract of fruiting bodies promoted cell proliferation in MCF-7 cells. Using bioassay-guided fractionation of the methanol extract and chemical investigation, we isolated and identified four steroids and four fatty acids from the active fraction. All eight compounds were evaluated by E-screen assay for their estrogen-like effects in MCF-7 cells. Among the tested isolates, only (3,5,22)-ergost-22-en-3-ol promoted cell proliferation in MCF-7 cells; this effect was mitigated by the ER antagonist, ICI 182,780. The mechanism underlying the estrogen-like effect of (3,5,22)-ergost-22-en-3-ol was evaluated using Western blot analysis to detect the expression of extracellular signal-regulated kinase (ERK), phosphatidylinositol 3-kinase (PI3K), Akt, and estrogen receptor α (ERα). We found that (3,5,22)-ergost-22-en-3-ol induced an increase in phosphorylation of ERK, PI3K, Akt, and ERα. Together, these experimental results suggest that (3,5,22)-ergost-22-en-3-ol is responsible for the estrogen-like effects of and may potentially aid control of estrogenic activity in menopause.
（Scop.）Sing.，一种属于 的蘑菇，已在传统东方医学中用于治疗胆囊炎，促进胆汁分泌和调节胆管压力。本研究使用雌激素受体阳性乳腺癌细胞系（MCF-7）中的细胞增殖测定法评估了 的雌激素样作用。我们发现 子实体的甲醇提取物可促进 MCF-7 细胞的增殖。通过对甲醇提取物进行基于生物测定的分级分离和化学研究，我们从活性部分分离并鉴定了四种甾体和四种脂肪酸。所有八种化合物均通过 E-screen assay 在 MCF-7 细胞中评估其雌激素样作用。在所测试的分离物中，只有（3,5,22）-麦角甾-22-烯-3-醇可促进 MCF-7 细胞的增殖；这种作用被 ER 拮抗剂 ICI 182,780 减轻。通过 Western blot 分析评估了（3,5,22）-麦角甾-22-烯-3-醇的雌激素样作用的机制，以检测细胞外信号调节激酶（ERK），磷酸肌醇 3-激酶（PI3K），Akt 和雌激素受体α（ERα）的表达。我们发现（3,5,22）-麦角甾-22-烯-3-醇诱导 ERK，PI3K，Akt 和 ERα 的磷酸化增加。综上所述，这些实验结果表明（3,5,22）-麦角甾-22-烯-3-醇是 的雌激素样作用的原因，并可能有助于控制更年期的雌激素活性。
