Faculty of Pharmacy, "Carol Davila" University of Medicine and Pharmacy of Bucharest, 37 Dionisie Lupu Street, Sector 2, 020021 Bucharest, Romania.
Faculty of Medicine and Pharmacy, "Dunarea de Jos" University of Galaţi, 35 A.I. Cuza Str., 800010 Galaţi, Romania.
Molecules. 2020 May 15;25(10):2321. doi: 10.3390/molecules25102321.
Identification and quantification of polyphenols in plant material are of great interest since they make a significant contribution to its total bioactivity. In the present study, an UPLC-Orbitrap-MS/MS approach using the variable data acquisition mode (DIA) was developed and applied for rapid separation, identification, and quantification of the main polyphenolic compounds in . and . sprouts in different germination stages. Based on accurate MS data and fragment ions identification strategy, a total of 29 compounds were identified by comparing their accurate masses, fragment ions, retention times, and literatures. Additionally, a number of 30 compounds were quantified by comparing to the reference standards. Data were statistically analysed. For both plant species, the sprouts of the third germination day are valuable sources of bioactive compounds and could be used in phytotherapy and nutrition. Although (Red Clover) is considered to be a reference for natural remedies in relieving menopause disorders, alfalfa also showed a high level of biological active compounds with estrogenic activity.
由于植物材料中的多酚对其总生物活性有重要贡献，因此对其进行鉴定和定量分析具有重要意义。本研究采用基于可变数据采集模式（DIA）的 UPLC-Orbitrap-MS/MS 方法，用于快速分离、鉴定和定量不同发芽阶段的 和 芽中的主要多酚类化合物。基于准确的 MS 数据和碎片离子鉴定策略，通过比较准确质量、碎片离子、保留时间和文献，共鉴定出 29 种化合物。此外，通过与对照品比较，定量了 30 种化合物。对数据进行了统计分析。对于这两个植物物种，发芽第三天的芽是生物活性化合物的有价值来源，可用于植物疗法和营养。虽然 （红三叶草）被认为是缓解更年期障碍的天然药物的参考，但紫花苜蓿也显示出具有雌激素活性的高生物活性化合物水平。
