Pacaud P, Loirand G, Mironneau C, Mironneau J
Laboratoire de Physiologie Cellulaire, Institut de Biochimie Cellulaire et Neurochimie du CNRS, Université de Bordeaux II, France.
Pflugers Arch. 1987 Nov;410(4-5):557-9. doi: 10.1007/BF00586539.
The effects of noradrenaline (NA) were investigated on both fast and slow Ca2+ currents in isolated vascular cells from rat portal vein in short-term primary culture using the whole-cell patch-clamp technique. NA (1 microM) stimulated the fast Ca2+ current when the slow Ca2+ current was inhibited either by application of a dihydropyridine derivative or by increasing the cytoplasmic Ca2+ concentration. Noradrenaline and caffeine greatly reduced the slow Ca2+ current. As this inhibitory effect is suppressed after depletion of the intracellular Ca2+ store, we propose that reduction of the slow Ca2+ current by noradrenaline is mediated by a Ca2+ release from the sarcoplasmic reticulum.
采用全细胞膜片钳技术，在短期原代培养的大鼠门静脉分离血管细胞中，研究了去甲肾上腺素（NA）对快、慢钙电流的影响。当慢钙电流通过应用二氢吡啶衍生物或增加细胞质钙浓度被抑制时，NA（1微摩尔）刺激快钙电流。去甲肾上腺素和咖啡因大大降低了慢钙电流。由于这种抑制作用在细胞内钙储存耗尽后被抑制，我们提出去甲肾上腺素对慢钙电流的降低是由肌浆网释放钙介导的。
