Kyathanahalli Chandrashekara Nagaraj, Manjunath Mallayya Jayawanth
Obstetrics & Gynecology, B330, Magee Women's Research Institute, University of Pittsburgh, 204 Craft Avenue, Pittsburgh, PA, 15213, USA,
Protoplasma. 2014 Sep;251(5):1021-9. doi: 10.1007/s00709-014-0612-5. Epub 2014 Feb 1.
Male reproductive dysfunctions and infertility are the common consequences of overt diabetes. Available evidence support oxidative stress to be the underlying mechanism for the manifestation of testicular complications during diabetes. In the present study, we assessed the attenuating effects of Withania somnifera root extract (WS) on diabetes-induced testicular oxidative disturbances in prepubertal rats. Four-week-old prepubertal rats were assigned into nondiabetic control, streptozotocin (STZ)-treated and STZ+WS supplemented (500 mg/kg b.w./d, oral, 15 days) groups. Experimental diabetes was induced by a single intraperitoneal injection of STZ (90 mg/kg b.w). Terminally, all animals were killed, and markers of oxidative stress were determined in the testis cytosol and mitochondrial fraction. Severe hyperglycemia and regression in testis size were apparent in diabetic rats. A decline in antioxidant defenses with subsequent elevation in the generation of reactive oxygen species and lipid peroxidation was discernible in testis cytosol and mitochondria of diabetic prepubertal rats, which was significantly reversed by WS. However, there was partial restoration of glucose-6-phosphate dehydrogenase, lactate dehydrogenase, and 3-beta hydroxysteroid dehydrogenase activities in testis of diabetic prepubertal rats administered with WS. Taken together, data accrued suggest the potential of WS to improve diabetes-induced testicular dysfunctions in prepubertal rats.
男性生殖功能障碍和不育是显性糖尿病的常见后果。现有证据支持氧化应激是糖尿病期间睾丸并发症表现的潜在机制。在本研究中，我们评估了印度人参根提取物（WS）对青春期前大鼠糖尿病诱导的睾丸氧化紊乱的减轻作用。将四周龄的青春期前大鼠分为非糖尿病对照组、链脲佐菌素（STZ）处理组和STZ+WS补充组（500mg/kg体重/天，口服，15天）。通过单次腹腔注射STZ（90mg/kg体重）诱导实验性糖尿病。最后，处死所有动物，并在睾丸细胞质和线粒体部分测定氧化应激标志物。糖尿病大鼠出现严重高血糖和睾丸大小缩小。在糖尿病青春期前大鼠的睾丸细胞质和线粒体中，抗氧化防御能力下降，随后活性氧生成和脂质过氧化增加，而WS可显著逆转这种情况。然而，给予WS的糖尿病青春期前大鼠睾丸中的葡萄糖-6-磷酸脱氢酶、乳酸脱氢酶和3-β羟基类固醇脱氢酶活性部分恢复。综上所述，积累的数据表明WS有改善青春期前大鼠糖尿病诱导的睾丸功能障碍的潜力。
