Tsuchiya Tomoshi, Sivarapatna Amogh, Rocco Kevin, Nanashima Atsushi, Nagayasu Takeshi, Niklason Laura E
Division of Surgical Oncology; Department of Surgery; Nagasaki University Graduate School of Biomedical Sciences; Nagasaki, Japan.
Departments of Anesthesia and Biomedical Engineering; Yale University; New Haven, CT USA.
Organogenesis. 2014 Apr-Jun;10(2):196-207. doi: 10.4161/org.27846. Epub 2014 Jan 31.
The shortage of donor lungs for transplantation causes a significant number of patient deaths. The availability of laboratory engineered, functional organs would be a major advance in meeting the demand for organs for transplantation. The accumulation of information on biological scaffolds and an increased understanding of stem/progenitor cell behavior has led to the idea of generating transplantable organs by decellularizing an organ and recellularizing using appropriate cells. Recellularized solid organs can perform organ-specific functions for short periods of time, which indicates the potential for the clinical use of engineered solid organs in the future. The present review provides an overview of progress and recent knowledge about decellularization and recellularization-based approaches for generating tissue engineered lungs. Methods to improve decellularization, maturation of recellularized lung, candidate species for transplantation and future prospects of lung bioengineering are also discussed.
用于移植的供体肺短缺导致大量患者死亡。实验室构建的功能性器官的可用性将是满足移植器官需求方面的一项重大进展。关于生物支架的信息积累以及对干细胞/祖细胞行为的深入理解，催生了通过使器官脱细胞并使用合适的细胞重新细胞化来生成可移植器官的想法。重新细胞化的实体器官能够在短时间内执行器官特异性功能，这表明未来工程化实体器官在临床应用方面具有潜力。 本综述概述了基于脱细胞和重新细胞化方法生成组织工程肺的进展和最新知识。还讨论了改善脱细胞效果的方法、重新细胞化肺的成熟、移植的候选物种以及肺生物工程的未来前景。
