Wang Yujia, Nicolas Clara T, Chen Harvey S, Ross Jeffery J, De Lorenzo Silvana B, Nyberg Scott L
Division of Transplant Surgery, Department of Surgery, Mayo Clinic, Rochester, MN, USA.
Cells Tissues Organs. 2017;203(4):203-214. doi: 10.1159/000452761. Epub 2016 Dec 29.
Liver transplantation from deceased or living human donors remains the only proven option for patients with end-stage liver disease. However, the shortage of donor organs is a significant clinical concern that has led to the pursuit of tissue-engineered liver grafts generated from decellularized liver extracellular matrix and functional cells. Investigative efforts on optimizing both liver decellularization and recellularization protocols have been made in recent decades. In the current review, we briefly summarize these advances, including the generation of high-quality liver extracellular matrix scaffolds, evaluation criteria for quality control, modification of matrix for enhanced properties, and reseeding strategies. These efforts to optimize the methods of decellularization and recellularization lay the groundwork towards generating a transplantable, human-sized liver graft for the treatment of patients with severe liver disease.
对于终末期肝病患者而言，来自已故或活体人类供体的肝移植仍是唯一经证实的治疗选择。然而，供体器官短缺是一个重大的临床问题，这促使人们寻求利用脱细胞肝细胞外基质和功能细胞构建的组织工程肝移植物。近几十年来，人们一直在努力优化肝脏脱细胞和再细胞化方案。在本综述中，我们简要总结了这些进展，包括高质量肝细胞外基质支架的制备、质量控制评估标准、基质改性以增强性能以及重新接种策略。这些优化脱细胞和再细胞化方法的努力为构建可移植的、适合人类大小的肝移植物以治疗重症肝病患者奠定了基础。
