Department of Polymer Science and Engineering & Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 34134, Korea.
Predictive Model Research Center, Korea Institute of Toxicology, Daejeon 34114, Korea.
Int J Mol Sci. 2021 Mar 2;22(5):2491. doi: 10.3390/ijms22052491.
The process of evaluating the efficacy and toxicity of drugs is important in the production of new drugs to treat diseases. Testing in humans is the most accurate method, but there are technical and ethical limitations. To overcome these limitations, various models have been developed in which responses to various external stimuli can be observed to help guide future trials. In particular, three-dimensional (3D) cell culture has a great advantage in simulating the physical and biological functions of tissues in the human body. This article reviews the biomaterials currently used to improve cellular functions in 3D culture and the contributions of 3D culture to cancer research, stem cell culture and drug and toxicity screening.
评估药物疗效和毒性的过程对于治疗疾病的新药生产非常重要。在人体中进行测试是最准确的方法，但存在技术和伦理方面的限制。为了克服这些限制，已经开发出各种模型，通过观察对各种外部刺激的反应来帮助指导未来的试验。特别是，三维（3D）细胞培养在模拟人体组织的物理和生物学功能方面具有很大的优势。本文综述了目前用于改善 3D 培养中细胞功能的生物材料，以及 3D 培养对癌症研究、干细胞培养和药物毒性筛选的贡献。
