合成聚合物通过减少解冻过程中的冰晶生长来实现非玻璃体细胞的冷冻保存。
Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing.
机构信息
1] Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK [2] Clinical Sciences Research Laboratories, University of Warwick, Clifford Bridge Road, Coventry CV2 2DX, UK [3] Molecular Organisation and Assembly in Cells (MOAC), Doctoral Training Centre, University of Warwick, Coventry CV4 7AL, UK.
1] Clinical Sciences Research Laboratories, University of Warwick, Clifford Bridge Road, Coventry CV2 2DX, UK [2].
出版信息
Nat Commun. 2014;5:3244. doi: 10.1038/ncomms4244.
The cryopreservation of cells, tissue and organs is fundamental to modern biotechnology, transplantation medicine and chemical biology. The current state-of-the-art method of cryopreservation is the addition of large amounts of organic solvents such as glycerol or dimethyl sulfoxide, to promote vitrification and prevent ice formation. Here we employ a synthetic, biomimetic, polymer, which is capable of slowing the growth of ice crystals in a manner similar to antifreeze (glyco)proteins to enhance the cryopreservation of sheep and human red blood cells. We find that only 0.1 wt% of the polymer is required to attain significant cell recovery post freezing, compared with over 20 wt% required for solvent-based strategies. These results demonstrate that synthetic antifreeze (glyco)protein mimics could have a crucial role in modern regenerative medicine to improve the storage and distribution of biological material for transplantation.
细胞、组织和器官的低温保存是现代生物技术、移植医学和化学生物学的基础。目前最先进的低温保存方法是添加大量的有机溶剂，如甘油或二甲基亚砜，以促进玻璃化并防止冰晶形成。在这里，我们采用了一种合成的、仿生的聚合物，它能够以类似于抗冻（糖）蛋白的方式减缓冰晶的生长，从而增强绵羊和人红细胞的低温保存。我们发现，与基于溶剂的策略所需的 20%以上的重量比相比，仅需 0.1%重量比的聚合物即可实现显著的细胞复苏。这些结果表明，合成的抗冻（糖）蛋白模拟物在现代再生医学中可能具有关键作用，可改善用于移植的生物材料的储存和运输。
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