Lucena Teresa, Ruvira María A, Macián M Carmen, Pujalte María J, Arahal David R
Colección Española de Cultivos Tipo (CECT) and Departamento de Microbiología y Ecología, Universitat de València, Valencia, Spain,
Antonie Van Leeuwenhoek. 2014 Apr;105(4):671-8. doi: 10.1007/s10482-014-0121-8. Epub 2014 Feb 1.
Strain 4SM10(T), an aerobic marine, Gram-negative, heterotrophic and non pigmented bacterium isolated from seawater from Vinaroz in Castellón, Spain, was characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence placed the strain within the Roseobacter clade in the family Rhodobacteraceae. Phylogenetic analyses also showed that strain 4SM10(T) forms a stable clade with species of the genus Roseovarius, being related to Roseovarius nubinhibens ISM(T) and Roseovarius aestuarii SMK-122(T) at 97.5 and 97.4 % 16S rRNA sequence similarity, respectively. Average Nucleotide Identity (ANI) values, determined as a measure of overall genomic resemblance, confirmed that strain 4SM10(T) does not belong to the same species as R. aestuarii CECT 7745(T) and Roseovarius nubinhibens CECT 7750(T) displaying ANI values well below the 95 % boundary for genomic species. Strain 4SM10(T) requires Na(+) plus a divalent cation (either Mg(2+) or Ca(2+)) to grow, reduces nitrate to nitrite and uses a large number of amino acids and organic acids (but no carbohydrates) as sole carbon sources. Enzymatic activities displayed in API ZYM tests are alkaline phosphatase, leucine arylamidase and acid phosphatase. The major cellular fatty acids were identified as C18:1 ω7c and/or C18:1 ω6c (67.1 %). The DNA G+C content was determined to be 54.27 mol%. Based on the genotypic and phenotypic data obtained, the name Roseovarius albus sp. nov. is proposed for this novel taxon, with the type strain 4SM10(T) (=CECT 7450(T) = KCTC 22653(T)).
菌株4SM10(T)是从西班牙卡斯特利翁省比纳罗斯的海水中分离出的一株需氧海洋革兰氏阴性异养非色素细菌，采用多相分类法对其进行了特征描述。16S rRNA基因序列分析将该菌株置于红杆菌科的玫瑰杆菌属分支内。系统发育分析还表明，菌株4SM10(T)与玫瑰变色菌属的物种形成一个稳定的分支，与抑制玫瑰变色菌ISM(T)和河口玫瑰变色菌SMK-122(T)的16S rRNA序列相似性分别为97.5%和97.4%。作为整体基因组相似性度量的平均核苷酸同一性(ANI)值证实，菌株4SM10(T)与河口玫瑰变色菌CECT 7745(T)和抑制玫瑰变色菌CECT 7750(T)不属于同一物种，其ANI值远低于基因组物种的95%界限。菌株4SM10(T)生长需要Na(+)加一种二价阳离子(Mg(2+)或Ca(2+))，能将硝酸盐还原为亚硝酸盐，并利用大量氨基酸和有机酸(但不利用碳水化合物)作为唯一碳源。API ZYM试验显示的酶活性为碱性磷酸酶、亮氨酸芳基酰胺酶和酸性磷酸酶。主要细胞脂肪酸鉴定为C18:1 ω7c和/或C18:1 ω6c(67.1%)。DNA G+C含量测定为54.27 mol%。基于所获得的基因型和表型数据，建议将这个新分类单元命名为白色玫瑰变色菌，模式菌株为4SM10(T)(=CECT 7450(T)=KCTC 22653(T))。
