Department of Botany, University of California, Berkeley.
Planta. 1971 Jun;101(2):95-100. doi: 10.1007/BF00387620.
A procedure is described for the isolation of large numbers of viable aleurone protoplasts. After treatment with "Onozuka" cellulase protoplasts were obtained which were surrounded by a thin, "Onozuka"-resistant wall. These cells were termed "spheroplasts". Treatment of spheroplasts with "Glusulase" digested away the residual wall, yielding naked protoplasts. Measurements of respiration using a Clarke-type oxygen electrode indicated that aleurone cells isolated by this procedure were viable.
描述了一种大规模分离活的糊粉层原生质体的方法。用“Onozuka”纤维素酶处理后，得到了被一层薄的、耐“Onozuka”的细胞壁包围的原生质体。这些细胞被称为“球形体”。用“Glusulase”处理球形体可以消化掉残留的细胞壁，得到裸露的原生质体。使用克拉克型氧电极测量呼吸表明，通过该程序分离的糊粉层细胞是有活力的。
