Choi Eunyoung, Roland Joseph T, Barlow Brittney J, O'Neal Ryan, Rich Amy E, Nam Ki Taek, Shi Chanjuan, Goldenring James R
Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee, USA Epithelial Biology Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Epithelial Biology Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Gut. 2014 Nov;63(11):1711-20. doi: 10.1136/gutjnl-2013-305964. Epub 2014 Jan 31.
The glands of the stomach body and antral mucosa contain a complex compendium of cell lineages. In lower mammals, the distribution of oxyntic glands and antral glands define the anatomical regions within the stomach. We examined in detail the distribution of the full range of cell lineages within the human stomach.
We determined the distribution of gastric gland cell lineages with specific immunocytochemical markers in entire stomach specimens from three non-obese organ donors.
The anatomical body and antrum of the human stomach were defined by the presence of ghrelin and gastrin cells, respectively. Concentrations of somatostatin cells were observed in the proximal stomach. Parietal cells were seen in all glands of the body of the stomach as well as in over 50% of antral glands. MIST1 expressing chief cells were predominantly observed in the body although individual glands of the antrum also showed MIST1 expressing chief cells. While classically described antral glands were observed with gastrin cells and deep antral mucous cells without any parietal cells, we also observed a substantial population of mixed type glands containing both parietal cells and G cells throughout the antrum.
Enteroendocrine cells show distinct patterns of localisation in the human stomach. The existence of antral glands with mixed cell lineages indicates that human antral glands may be functionally chimeric with glands assembled from multiple distinct stem cell populations.
胃体和胃窦黏膜的腺体包含复杂的细胞谱系集合。在低等哺乳动物中，泌酸腺和胃窦腺的分布界定了胃内的解剖区域。我们详细研究了人类胃内全谱系细胞的分布情况。
我们使用特定免疫细胞化学标志物，在来自三名非肥胖器官捐赠者的全胃标本中确定胃腺细胞谱系的分布。
人类胃的解剖学上的体部和窦部分别由胃饥饿素和胃泌素细胞的存在来界定。在胃近端观察到生长抑素细胞的聚集。壁细胞可见于胃体的所有腺体以及超过50%的胃窦腺。表达MIST1的主细胞主要见于胃体，尽管胃窦的个别腺体也显示有表达MIST1的主细胞。虽然经典描述的胃窦腺含有胃泌素细胞和深层胃窦黏液细胞而无任何壁细胞，但我们也在整个胃窦中观察到大量同时含有壁细胞和G细胞的混合型腺体。
肠内分泌细胞在人类胃中显示出独特的定位模式。具有混合细胞谱系的胃窦腺的存在表明，人类胃窦腺在功能上可能是由多个不同干细胞群体组装而成的嵌合体。
