Nisa C, Kitamura N, Sasaki M, Agungpriyono S, Choliq C, Budipitojo T, Yamada J, Sigit K
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor 16680, Indonesia.
Anat Histol Embryol. 2005 Dec;34(6):373-8. doi: 10.1111/j.1439-0264.2005.00626.x.
The distribution and relative frequency of six kinds of endocrine cells in the stomach of the Malayan pangolin, Manis javanica were studied immunohistochemically using the avidin-biotin-peroxidase complex method. The stomach of the pangolin has three regions of mucous gland, one oxyntic gland and one pyloric gland. Cells immunoreactive for chromogranin, serotonin, somatostatin, BPP and glucagon were detected in all of the gastric glands, while gastrin-immunoreactive cells were found in the entire gastric gland except for the oxyntic gland. The distribution pattern of endocrine cells in the mucous gland and pyloric gland was mainly from the middle to apical portions of the glands. The endocrine cells were rare or not detected in the basal portion of all of the mucous glands and pyloric gland, but they were also found in the basal portion of the oxyntic gland. The distribution pattern of the endocrine cells in the mucous and pyloric glands suggested that this position facilitates a quick response to the luminal ingesta. The wide distribution of gastrin-immunoreactive cells in all of the mucous glands and pyloric gland was the most remarkable finding. This distribution suggests a major function of gastrin-immunoreactive cells for the digestive process in the Malayan pangolin stomach.
采用抗生物素蛋白-生物素-过氧化物酶复合物法，对马来穿山甲（Manis javanica）胃内六种内分泌细胞的分布及相对频率进行了免疫组织化学研究。穿山甲的胃有三个黏液腺区域、一个泌酸腺和一个幽门腺。在所有胃腺中均检测到嗜铬粒蛋白、5-羟色胺、生长抑素、胰多肽和胰高血糖素免疫反应性细胞，而胃泌素免疫反应性细胞在除泌酸腺外的整个胃腺中均有发现。黏液腺和幽门腺内分泌细胞的分布模式主要是从腺体的中部到顶端部分。在所有黏液腺和幽门腺的基部，内分泌细胞很少或未被检测到，但在泌酸腺的基部也发现了内分泌细胞。黏液腺和幽门腺内分泌细胞的分布模式表明，这个位置有利于对腔内食糜做出快速反应。胃泌素免疫反应性细胞在所有黏液腺和幽门腺中的广泛分布是最显著的发现。这种分布表明胃泌素免疫反应性细胞在马来穿山甲胃的消化过程中具有主要功能。
