Dunk A A, Novick D, Thomas H C
Academic Dept. of Medicine, Royal Free Hospital School of Medicine, London, U.K.
Scand J Gastroenterol. 1987 Dec;22(10):1245-50. doi: 10.3109/00365528708996471.
We have measured natural killer (NK) cell activity in patients with hepatocellular carcinoma (HCC) and have examined the effects of in vitro and in vivo administration of alpha-interferon (IFN) on NK cell activity. The NK cell cytotoxicity of HCC patients was significantly lower than that of patients with cirrhosis or healthy controls. Reduced NK cell cytotoxicity in HCC did not correlate significantly with either the serum alpha-foetoprotein concentration or the patient WHO performance grade. NK cell cytotoxicity in all groups could be increased by prior incubation of effector cells with IFN, but this was significant only in HCC patients, in whom 10 IU/ml of IFN increased NK cell cytotoxicity from 37 +/- 10% to 53 +/- 8% (effector to target ratio, 50:1, mean +/- SEM; p less than 0.05). Further increases in IFN concentration failed to increase NK cell activity further. NK cell cytotoxicity was measured immediately before and 24 h after 2.5 x 10(6) IU/m2 of IFN was given subcutaneously to four HCC patients. NK cell cytotoxicity rose from 27 +/- 9% to 61 +/- 5% (effector to target ratio, 50:1, mean +/- SEM; p = 0.05).
我们检测了肝细胞癌（HCC）患者的自然杀伤（NK）细胞活性，并研究了体外及体内给予α干扰素（IFN）对NK细胞活性的影响。HCC患者的NK细胞细胞毒性显著低于肝硬化患者或健康对照。HCC患者NK细胞细胞毒性降低与血清甲胎蛋白浓度或患者的世界卫生组织体能状况分级均无显著相关性。通过效应细胞与IFN预先孵育，所有组的NK细胞细胞毒性均可增加，但仅在HCC患者中显著，其中10 IU/ml的IFN可使NK细胞细胞毒性从37±10%增加至53±8%（效应细胞与靶细胞比例为50:1，均值±标准误；p<0.05）。IFN浓度进一步增加未能进一步提高NK细胞活性。对4例HCC患者皮下给予2.5×10(6) IU/m2的IFN，在给药前及给药后24小时立即检测NK细胞细胞毒性。NK细胞细胞毒性从27±9%升至61±5%（效应细胞与靶细胞比例为50:1，均值±标准误；p = 0.05）。
