慢性肝病和肝细胞癌中自然杀伤（NK）细胞活性及其对α（Le）干扰素的体外反应。

Natural killer (NK) cell activity and its in vitro response to interferon-alpha(Le) in chronic liver diseases and hepatocellular carcinoma.

作者信息

Hirai N, Kato Y, Kobayashi K, Hattori N

出版信息

Liver. 1986 Aug;6(4):212-20. doi: 10.1111/j.1600-0676.1986.tb01068.x.

DOI:10.1111/j.1600-0676.1986.tb01068.x

PMID:3022099

Abstract

The natural killer (NK) cell activity of peripheral blood lymphocytes (PBL) in patients with various chronic liver diseases, and its in vitro response to human interferon-alpha(Le) were investigated using a 16-h 51-Cr releasing cytotoxicity assay against YAC-1 or RSa target cells. The NK cell activity was found to be higher in chronic active hepatitis (CAH) and liver cirrhosis (LC) patients without HCC, whereas it was slightly lower in LC patients with hepatocellular carcinoma (HCC), than in normal controls. By the addition of IFN-alpha(Le) in vitro, the NK cell activity was clearly and dose-dependently augmented, even in chronic liver diseases, as well as in normal controls. The magnitude of this augmentation by 10,000 IU/ml of IFN-alpha(Le) in the various chronic liver diseases was not significantly different from that in normal controls. The results suggested that the response of NK cells to IFN-alpha(Le) is not impaired even in chronic liver disease conditions, while the level of NK cell activity may vary according to the type of chronic liver disease and may decrease in patients with HCC.

摘要

采用针对YAC-1或RSa靶细胞的16小时51铬释放细胞毒性试验，研究了各种慢性肝病患者外周血淋巴细胞（PBL）的自然杀伤（NK）细胞活性及其对人α-干扰素（Le）的体外反应。发现无肝癌的慢性活动性肝炎（CAH）和肝硬化（LC）患者的NK细胞活性高于正常对照组，而有肝细胞癌（HCC）的LC患者的NK细胞活性略低于正常对照组。通过在体外添加α-干扰素（Le），即使在慢性肝病患者以及正常对照组中，NK细胞活性也明显且呈剂量依赖性增强。在各种慢性肝病中，10000 IU/ml的α-干扰素（Le）引起的这种增强幅度与正常对照组无显著差异。结果表明，即使在慢性肝病情况下，NK细胞对α-干扰素（Le）的反应也未受损，而NK细胞活性水平可能因慢性肝病类型而异，且在HCC患者中可能降低。