绵羊对羊口疮病毒感染的体液抗体反应的定性和定量评估。

A qualitative and quantitative assessment of the humoral antibody response of the sheep to orf virus infection.

作者信息

McKeever D J, Reid H W, Inglis N F, Herring A J

机构信息

Moredun Research Institute, Edinburgh, Gt. Britain.

出版信息

Vet Microbiol. 1987 Nov;15(3):229-41. doi: 10.1016/0378-1135(87)90077-0.

DOI:10.1016/0378-1135(87)90077-0

PMID:2448946

Abstract

The serological response of naturally and experimentally infected lambs to orf virus infection was analysed using an enzyme-linked immunosorbent assay (ELISA) together with the Western blotting technique. The combination of these two methods permitted a qualitative and quantitative assessment of the response, which revealed considerable variation between animals. Despite this, all post-exposure sera reacted with a polypeptide (molecular weight 40 kilodaltons) which appears to be a component of the surface tubules that are characteristic of the virus.

摘要

利用酶联免疫吸附测定法（ELISA）和蛋白质印迹技术，分析了自然感染和实验感染的羔羊对羊口疮病毒感染的血清学反应。这两种方法的结合能够对反应进行定性和定量评估，结果显示不同动物之间存在显著差异。尽管如此，所有暴露后血清均与一种多肽（分子量40千道尔顿）发生反应，该多肽似乎是该病毒特有的表面小管的一个组成部分。