Yamamoto Y, Hiranuma Y, Eba M, Okitsu M, Utsumi N, Tajima Y, Tatemoto Y, Mori M
Department of Oral Surgery, Josai Dental University, Saitama, Japan.
Virchows Arch A Pathol Anat Histopathol. 1988;412(3):189-96. doi: 10.1007/BF00737142.
Calcifying odontogenic cysts (COC) were immunohistochemically described using different keratin proteins and involucrin as well as histopathology. The cystic lining epithelium was composed of calcifying, keratinizing, squamous, and columnar epithelial cells, and included calcified masses of irregular shape and various size as well as ghost cells. Calcifying epithelium gave negative or only trace staining for keratins detected with low molecular keratin (PKK1), but were regularly positive with high molecular keratin (KL1) and polyclonal antibody for keratin (TK). They were occasionally positive for involucrin. The cells located in the periphery of the calcified masses had a particular abundance of high molecular weight and total keratins (KL1 and TK). Calcified bodies and ghost cells were devoid of any immunoreactivity. Squamous epithelium was relatively similar to that of normal squamous cell epithelium in the oral mucosa. It were most commonly found in columnar cystic epithelial cells which displayed intense staining with all immunoreagents. It is postulated that such epithelial cells may have a strong potentiality to transform into ghost cells or to undergo metaplasia. They may develop altered synthesis of homogenous acellular materials and finally become transformed into calcifying epithelium containing dystrophic calcified masses.
采用不同的角蛋白和外皮蛋白以及组织病理学方法，对牙源性钙化囊肿（COC）进行了免疫组织化学描述。囊肿衬里上皮由钙化、角化、鳞状和柱状上皮细胞组成，包括形状不规则、大小各异的钙化团块以及影细胞。钙化上皮对低分子量角蛋白（PKK1）检测的角蛋白呈阴性或仅呈微量染色，但对高分子量角蛋白（KL1）和角蛋白多克隆抗体（TK）呈规律性阳性。它们偶尔对外皮蛋白呈阳性。位于钙化团块周边的细胞特别富含高分子量角蛋白和总角蛋白（KL1和TK）。钙化小体和影细胞无任何免疫反应性。鳞状上皮与口腔黏膜正常鳞状细胞上皮相对相似。最常见于柱状囊性上皮细胞，这些细胞对所有免疫试剂均呈强烈染色。据推测，此类上皮细胞可能具有转化为影细胞或发生化生的强大潜能。它们可能会出现均质无细胞物质合成改变，最终转化为含有营养不良性钙化团块的钙化上皮。
