Zhang Zhi-hai, Fang Rong, Yu Bin, Liu Zhi-shuang, Zhao Ze-jun, Xu Xin-wen, Huang Xiu-long, Jia Tie-wu
Hanchuan Institute of Schistosomiasis Prevention and Control, Hubei Province, Hanchuan 431600, China.
National Institute of Parasitic Diseases, Chinese Centre for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2013 Oct;25(5):481-4.
To evaluate the effect of a novel plant molluscicide "Luo-wei" (Tea-seed distilled saponins, TDS) against Oncomelania hupensis in the field of lake areas in Hanchuan City, Hubei Province.
Immersing experiment and spraying experiment were carried out in 5 ditches and a beach land respectively in Hanchuan City, Hubei Province to compare the molluscicidal effects among 4% TDS, 50% wettable powder of niclosamide ethanolamine salt (WPN), and 26% suspension concentrate of metaldehyde and niclosamide (MNSC) by different dosages and time. The chi2 test or Fish's exact test was used to examine the differences of snail death rates among them.
After 72 h of immersing experiment, the snail death rates in the 2.5 g/m3 TDS group, 3.0 g/m3 TDS group, 2.0 g/m3 WPN group and 2.0 g/m3 MNSC group were 99.33%, 100%, 100%, and 100%, respectively, and there was no significant difference among them (P > 0.05). By the end of 15 d after the spraying experiments, the snail death rates were 91.86% in the 2.0 g/m2 MNSC group, 90.26% in the 5.0 g/m2 TDS group, 87.45% in the 2 g/m2 WPN group, and 86.10% in the 3 g/m2 TDS group. The differences between the 5.0 g/m2 TDS group and 2.0 g/m2 MNSC group, as well as the 3.0 g/m2 TDS group and the 2.0 g/m2 WPN group had no statistical significance (both P > 0.05).
The molluscicidal effect of TDS is similar to that of WPN or MNSC. The recommended dosage of TDS is 2.5 g/m3 for immersing or 5 g/ m2 for spraying in the field.
评估新型植物杀螺剂“罗威”（茶籽蒸馏皂苷，TDS）在湖北省汉川市湖区现场对日本血吸虫中间宿主钉螺的杀灭效果。
分别在湖北省汉川市的5条沟渠和1块滩地进行浸杀试验和喷洒试验，比较4% TDS、50%氯硝柳胺乙醇胺盐可湿性粉剂（WPN）和26% 四聚乙醛与氯硝柳胺悬浮剂（MNSC）不同剂量和时间的杀螺效果。采用卡方检验或Fisher精确检验分析它们之间钉螺死亡率的差异。
浸杀试验72小时后，2.5 g/m3 TDS组、3.0 g/m3 TDS组、2.0 g/m3 WPN组和2.0 g/m3 MNSC组的钉螺死亡率分别为99.33%、100%、100%和100%，差异无统计学意义（P>0.05）。喷洒试验15天后，2.0 g/m2 MNSC组钉螺死亡率为91.86%，5.0 g/m2 TDS组为90.26%，2 g/m2 WPN组为87.45%，3 g/m2 TDS组为86.10%。5.0 g/m2 TDS组与2.0 g/m2 MNSC组、3.0 g/m2 TDS组与2.0 g/m2 WPN组之间差异无统计学意义（均P>0.05）。
TDS的杀螺效果与WPN或MNSC相似。现场浸杀推荐剂量为2.5 g/m3，喷洒推荐剂量为5 g/m2。
