Wei Feng-hua, Xu Xing-jian, Yuan Yi, Xia Jing, Li Gui-ling, Liu Min, Chen Ru-juan, Zhou Zheng-ping, Dong Xing-qi, Feng Xi-guang, Qiu Dong-chuan, Chen Lin, Yi Ping, Wei Wang-yuan, Chen Hong-gen, Liu Hong-yun, Wang Tian-ping, Guo Jian-duo, Liang You-sheng, Dai Jian-rong, Wen Li-yong, Yan Xiao-lan
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2015 Aug;27(4):335-42.
To evaluate the effects of a novel molluscicide, the salt quinoid-2', 5-dichloro-4'-nitrosalicylanilide from niclosamide (LDS), with 10% wettable powder, in main schistosomiasis epidemic areas of China, including Hunan, Jiangxi, Hubei, Anhui, Jiangsu, Sichuan, Yunnan and Zhejiang Province.
In the immersion test, 6 effective concentrations of 10% LDS were tested respectively: 0.1, 0.2, 0.4, 0.6, 0.8, 1.0 g/m3 in the field; at the same time, 50% wettable powder of niclosamide ethanolamine salt (WPN) with effective concentrations of 1.0 g/m was used as the molluscicide control, and the fresh water as the blank control, then the mortality rates of 0. hupensis snails were recorded at 24 h, 48 h and 72 h after the immersion. In the spraying test and powder-spraying test, 5 effective dosages of 10% LDS were tested respectively: 0.2, 0.4, 0.6, 0.8, 1.0 g/m2, while 50% WPN 1.0 g/m2 was used as the molluscicide control, and the fresh water as the blank control in the field for 1 d, 3 d and 7 d, then the mortality rates of O. hupensis snails were recorded at 1 d, 3 d and 7 d after the spraying and powder-spraying.
The snail mortality rates of LDS using the immersion test for 72 h were more than 95% in the field of eight provinces (0.1 g/m in Sichuan, Jiangxi, Jiangsu and Zhejiang provinces, 0.2 g/m3 in Yunnan, Hunan and Hubei provinces, and 0.4 g/min Anhui Province); the snail mortality rates of LDS using the spraying test for 7 d were more than 85% (0.2 g/m2 in Hunan, Hubei, Jiangxi and Zhejiang provinces, 0.4 g/m2 in Sichuan and Anhui provinces, 0.6 g/m2 in Yunnan and Jiangsu provinces). The snail mortality rates of LDS the powder-spraying test for 7 d were more than 85% (0.6 g/m2 in Yunnan, Sichuan, Hubei, Jiangxi, Anhui, Jiangsu and Zhejiang provinces). According to the standards of "Efficacy test methods and evaluation of molluscicide for pesticide registration (NY/T 1617-2008)", LDS is a qualified molluscicide.
LDS has good molluscicidal effects through the immersion, spraying and powder-spraying test in the fields. It is suitable for a variety of environments to control O. hupensis snails of schistosomiasis endemic areas in China. The recommended dosages of LDS are 0.1-0.2 g/m3 by the immersion method, 0.2-0.4 g/m2 by the spraying method, and 0.4-0.6 g/m2 by the powder-spraying method in the fields.
评价新型杀螺剂——来自氯硝柳胺的盐醌类化合物2',5-二氯-4'-亚硝基水杨酰苯胺（LDS）10%可湿性粉剂在中国血吸虫病主要流行区（包括湖南、江西、湖北、安徽、江苏、四川、云南和浙江省）的效果。
在浸杀试验中，分别测试10% LDS的6个有效浓度：田间0.1、0.2、0.4、0.6、0.8、1.0 g/m³；同时，以有效浓度为1.0 g/m的氯硝柳胺乙醇胺盐50%可湿性粉剂（WPN）作为杀螺剂对照，以淡水作为空白对照，浸杀后24 h、48 h和72 h记录湖北钉螺的死亡率。在喷洒试验和喷粉试验中，分别测试10% LDS的5个有效剂量：0.2、0.4、0.6、0.8、1.0 g/m²，以50% WPN 1.0 g/m²作为杀螺剂对照，以淡水作为空白对照，在田间喷洒和喷粉后1 d、3 d和7 d记录湖北钉螺的死亡率。
八省田间浸杀试验72 h时LDS的钉螺死亡率均超过95%（四川省、江西省、江苏省和浙江省为0.1 g/m，云南省、湖南省和湖北省为0.2 g/m³，安徽省为0.4 g/min）；喷洒试验7 d时LDS的钉螺死亡率超过85%（湖南省、湖北省、江西省和浙江省为0.2 g/m²，四川省和安徽省为0.4 g/m²，云南省和江苏省为0.6 g/m²）。喷粉试验7 d时LDS的钉螺死亡率超过85%（云南省、四川省、湖北省、江西省、安徽省、江苏省和浙江省为0.6 g/m²）。按照《农药登记用杀螺剂药效试验方法及评价（NY/T 1617—2008）》标准，LDS为合格杀螺剂。
LDS通过田间浸杀、喷洒和喷粉试验显示有良好的杀螺效果。适用于多种环境，可用于控制中国血吸虫病流行区的湖北钉螺。田间推荐剂量浸杀法为0.1 - 0.2 g/m³，喷洒法为0.2 - 0.4 g/m²，喷粉法为0.
