Zhou Ning, Wang Qing-Ping, Jin Xing-Fang, Hou Zong-Liu, Peng Bao-Kun, Dan Qi-Qin, Wang Tin-Hua
Institute of Neuroscience, Kunming Medical University, Kunming 650031, China.
Institute of Neurological Disease and Translation Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 Nov;44(6):931-4.
OBJECTITVE: To investigate the effect of human umbilici mesenchymal cells (HUMSCs) implantation on the brain derived neurotrophic factor (BDNF) expression in diabetic foot rats.
SD rats were divided into three groups (n = 12): normal group, diadetic foot model group and HUMSC treatment group. Diabetic foot model in rats was established, then prepared HUMSC were implanted on the diabetic foot ulcers in rats, and control ones were administrated with saline only. The area of ulceration, sensory function, BDNF expression and its localization were determined by using morphology, physiological function measurement, RT-PCR and immunohistochemistry assay.
Siglificantly decreased area of ulceration in diabetic foot rats of HUMSC implantation group was observed. This was simultaneously companied with the sensory function improvement (P < 0.05). RT-PCR showed that BDNF mRNA expression was significantly up regulated (P < 0.05). BDNF immunstaining was located in epithelia tissue and the protein level of BDNF was markedly increased (P < 0.05).
HUMSC implantation maybe an effective strategy on the treatment of ulceration in diabetic foot rats, and the possible mechanism may involve in BDNF expression.
目的：探讨人脐间充质干细胞（HUMSCs）植入对糖尿病足大鼠脑源性神经营养因子（BDNF）表达的影响。
将SD大鼠分为三组（n = 12）：正常组、糖尿病足模型组和HUMSC治疗组。建立大鼠糖尿病足模型，然后将制备好的HUMSCs植入大鼠糖尿病足溃疡处，对照组仅给予生理盐水。通过形态学、生理功能测量、RT-PCR和免疫组化分析来确定溃疡面积、感觉功能、BDNF表达及其定位。
观察到HUMSC植入组糖尿病足大鼠的溃疡面积显著减小。同时伴有感觉功能改善（P < 0.05）。RT-PCR显示BDNF mRNA表达显著上调（P < 0.05）。BDNF免疫染色位于上皮组织，且BDNF蛋白水平明显升高（P < 0.05）。
HUMSC植入可能是治疗糖尿病足大鼠溃疡的有效策略，其可能机制可能与BDNF表达有关。
