软玻璃膜片吸管诱导的钾通道“失活”
Potassium channel "inactivation" induced by soft-glass patch pipettes.
作者信息
Cota G, Armstrong C M
机构信息
Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia 19104-6085.
出版信息
Biophys J. 1988 Jan;53(1):107-9. doi: 10.1016/S0006-3495(88)83071-6.
Abstract
We have studied the potassium currents of rat pituitary pars intermedia cells kept in primary culture using whole-cell recording with patch pipettes. The potassium current recorded with hard-glass pipettes is mainly carried by voltage-dependent channels that show slow inactivation in the presence of 0.5 mM internal EGTA. Fast "inactivation" of the potassium current is seen with patch pipettes fabricated from soft glass (soda glass or potash lead glass), and is probably caused by block of the potassium channels by di- or multivalent cations released from the glass.
摘要
我们使用膜片移液器进行全细胞记录，研究了原代培养的大鼠垂体中间部细胞的钾电流。用硬玻璃移液器记录的钾电流主要由电压依赖性通道携带，在存在0.5 mM内部乙二醇双四乙酸（EGTA）的情况下，这些通道表现出缓慢失活。用软玻璃（钠玻璃或钾铅玻璃）制成的膜片移液器可观察到钾电流的快速“失活”，这可能是由玻璃释放的二价或多价阳离子阻断钾通道所致。
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