Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Anatomical Sciences, Paramedical school, Guilan University of Medical Sciences, Langeroud, Iran.
Iran J Basic Med Sci. 2013 Nov;16(11):1163-9.
Platelet-rich plasma (PRP) has recently emerged as a promising strategy in regenerative medicine due to its multiple endogenous growth factors. Little is known about the role of PRP as a promoter in chondrogenesis of human adipose derived stem cells (hADSCs). The aim of this study was to determine whether PRP may be considered as a natural and easy achievable source of growth factors to promote the chondrogenic differentiation of hADSCs in Transwell culture. Materials and Methods : Biochemical, immunohistological and molecular assays were used to evaluate the effect of different concentrations (5%, 10%, and 15%) of PRP on chondrogenic differentiation of hADSCs in Transwell culture. Results : The cells in the presence of 10% PRP produced markedly higher amounts of GAG and DNA, in comparison to the control group. PRP also increased chondrogenic markers in these cells, such as sox-9, aggrecan and collagen type II. A high expression level of collagen type X as a hypertrophic marker was observed in cartilage produced by using either PRP or TGF-β1. Conclusion : Our findings indicate that autologous PRP at an optimum concentration had beneficial effects on differentiation of hADSCs in Transwell culture. Further, in vivo studies are necessary to fully define the clinical implications of PRP.
富血小板血浆（PRP）由于其多种内源性生长因子，最近在再生医学中成为一种很有前途的策略。关于 PRP 作为人脂肪来源干细胞（hADSCs）软骨形成的促进剂的作用知之甚少。本研究旨在确定 PRP 是否可以被认为是一种天然且易于获得的生长因子来源，以促进 hADSCs 在 Transwell 培养中的软骨分化。
使用生化、免疫组织化学和分子测定法来评估不同浓度（5%、10%和 15%）的 PRP 对 Transwell 培养中 hADSCs 软骨分化的影响。
与对照组相比，在 10% PRP 存在的情况下，细胞产生的 GAG 和 DNA 明显更多。PRP 还增加了这些细胞中的软骨形成标志物，如 Sox-9、聚集蛋白聚糖和 II 型胶原。在使用 PRP 或 TGF-β1 产生的软骨中观察到作为肥大标志物的高水平胶原 X。
我们的研究结果表明，自体 PRP 在最佳浓度下对 hADSCs 在 Transwell 培养中的分化有有益的影响。此外，还需要进行体内研究来充分定义 PRP 的临床意义。
