Basiri Arefeh, Hashemibeni Batool, Kazemi Mohammad, Valiani Ali, Aliakbari Maryam, Ghasemi Nazem
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Dent Res J (Isfahan). 2020 Jan 21;17(1):54-59. eCollection 2020 Jan-Feb.
The use of stem cells, growth factors, and scaffolds to repair damaged tissues is a new idea in tissue engineering. The aim of the present study is the investigation of Avocado/soybean (A/S) effects on chondrogenic differentiation of human adipose-derived stem cells (hADSCs) in micromass culture to access cartilage tissue with high quality.
In this an experimental study After hADSCs characterization, chondrogenic differentiation was induced using transforming growth factor beta 1 (TGF-β1) (10 ng/ml) and different concentrations (5, 10, and 20 μg/ml) of A/S in micromass culture. The efficiency of A/S on specific gene expression (types I, II, and X collagens, SOX9, and aggrecan) was evaluated using quantitative polymerase chain reaction. In addition, histological study was done using hematoxylin and eosin and toluidine blue staining all data were analyzed using one-way analysis of variance (ANOVA) and ≤ 0.05 was considered to be statistically significant.
The results of this study indicated that A/S can promote chondrogenic differentiation in a dose-dependent manner. In particular, 5 ng/ml A/S showed the highest expression of type II collagen, SOX9, and aggrecan which are effective and important markers in chondrogenic differentiation. In addition, the expression of types I and X collagens which are hypertrophic and fibrous factors in chondrogenesis is lower in present of 5 ng/ml A/S compared with TGF-β1 group ( ≤ 0.05). Moreover, the sulfated glycosaminoglycans in the extracellular matrix and the presence of chondrocytes within lacuna were more prominent in 5 ng/ml A/S group than other groups.
It can be concluded that A/S similar to TGF-β1 is able to facilitate the chondrogenic differentiation of hADSCs and do not have adverse effects of TGF-β1. Thus, TGF-β1 can be replaced by A/S in the field of tissue engineering.
利用干细胞、生长因子和支架修复受损组织是组织工程中的一个新想法。本研究的目的是研究鳄梨/大豆（A/S）对人脂肪来源干细胞（hADSCs）在微团培养中软骨形成分化的影响，以获得高质量的软骨组织。
在本实验研究中，对hADSCs进行表征后，在微团培养中使用转化生长因子β1（TGF-β1）（10 ng/ml）和不同浓度（5、10和20 μg/ml）的A/S诱导软骨形成分化。使用定量聚合酶链反应评估A/S对特定基因表达（I、II和X型胶原蛋白、SOX9和聚集蛋白聚糖）的效率。此外，使用苏木精和伊红以及甲苯胺蓝染色进行组织学研究，所有数据均使用单因素方差分析（ANOVA）进行分析，P≤0.05被认为具有统计学意义。
本研究结果表明，A/S可以以剂量依赖的方式促进软骨形成分化。特别是，5 ng/ml A/S显示出II型胶原蛋白、SOX9和聚集蛋白聚糖的最高表达，这些是软骨形成分化中有效且重要的标志物。此外，与TGF-β1组相比，在5 ng/ml A/S存在下，软骨形成中肥大和纤维因子I型和X型胶原蛋白的表达较低（P≤0.05）。此外，5 ng/ml A/S组细胞外基质中的硫酸化糖胺聚糖和陷窝内软骨细胞的存在比其他组更明显。
可以得出结论，A/S与TGF-β1相似，能够促进hADSCs的软骨形成分化，且没有TGF-β1的不良反应。因此，在组织工程领域，TGF-β1可以被A/S替代。
