Plant Signal Behav. 2013 Oct;8(10):doi: 10.4161/psb.26784. doi: 10.4161/psb.26784.
RAR 1 is identified as a critical protein involved in plant innate immunity. We investigated the role of RAR 1 in Agrobacterium-mediated plant transformation based on the previous findings that accessory proteins associated with the E3 ligase complex such as SGT1, which tightly interacts with RAR 1, play a role in the transformation process. RAR1 gene silencing in Nicotiana benthamiana and Arabidopsis rar1 mutant analyses suggested that RAR1 is required for early stages of Agrobacterium-mediated plant transformation. This finding further illustrates that RAR 1, along with SGT1, that serve as a HSP90 co-chaperone is important for Agrobacterium-mediated plant transformation.
RAR1 被鉴定为参与植物先天免疫的关键蛋白。基于先前的发现，即与 E3 连接酶复合物相关的辅助蛋白（如与 RAR1 紧密相互作用的 SGT1）在转化过程中发挥作用，我们研究了 RAR1 在农杆菌介导的植物转化中的作用。在烟草原生质体和拟南芥 rar1 突变体分析中沉默 RAR1 表明，RAR1 是农杆菌介导的植物转化早期所必需的。这一发现进一步表明，RAR1 与 SGT1 一起作为 HSP90 共伴侣，对于农杆菌介导的植物转化很重要。
