Liu Fuquan, Ni Weimin, Griffith Megan E, Huang Zhiyuan, Chang Changqing, Peng Wen, Ma Hong, Xie Daoxin
Laboratory of Plant Signal Transduction, Institute of Molecular and Cell Biology, 117609 Republic of Singapore.
Plant Cell. 2004 Jan;16(1):5-20. doi: 10.1105/tpc.017772. Epub 2003 Dec 19.
The requirement of CUL1 for Arabidopsis embryogenesis suggests that Skp1-CUL1-F-box protein (SCF) complexes play important roles during embryo development. Among the 21 Arabidopsis Skp1-like genes (ASKs), it is unknown which ASK gene(s) is essential for embryo development. In this study, we demonstrate a vital role for ASK1 and ASK2 in Arabidopsis embryogenesis and postembryonic development through analysis of the ask1 ask2 double mutant. Our detailed analysis indicates that the double mutations in both ASK1 and ASK2 affect cell division and cell expansion/elongation and cause a developmental delay during embryogenesis and lethality in seedling growth. The expression patterns of ASK1 and ASK2 were examined further and found to be consistent with their roles in embryogenesis and seedling development. We propose that mutations in ASK1 and ASK2 abolish all of the ASK1- and ASK2-based SCF and non-SCF complexes, resulting in alteration of gene expression and leading to defects in growth and development.
拟南芥胚胎发生对CUL1的需求表明，Skp1-CUL1-F-box蛋白（SCF）复合物在胚胎发育过程中发挥重要作用。在21个拟南芥Skp1样基因（ASKs）中，尚不清楚哪个ASK基因对胚胎发育至关重要。在本研究中，我们通过对ask1 ask2双突变体的分析，证明了ASK1和ASK2在拟南芥胚胎发生和胚后发育中的重要作用。我们的详细分析表明，ASK1和ASK2中的双突变影响细胞分裂以及细胞扩张/伸长，并在胚胎发生过程中导致发育延迟以及幼苗生长中的致死性。进一步检测了ASK1和ASK2的表达模式，发现与它们在胚胎发生和幼苗发育中的作用一致。我们提出，ASK1和ASK2中的突变消除了所有基于ASK1和ASK2的SCF和非SCF复合物，导致基因表达改变并导致生长和发育缺陷。
