分子伴侣和共分子伴侣Hsp90、RAR1和SGT1对本氏烟草中由青枯雷尔氏菌引起的青枯病具有负调控作用。
Molecular chaperons and co-chaperons, Hsp90, RAR1, and SGT1 negatively regulate bacterial wilt disease caused by Ralstonia solanacearum in Nicotiana benthamiana.
作者信息
Ito Makoto, Ohnishi Kouhei, Hikichi Yasufumi, Kiba Akinori
机构信息
a Laboratory of Plant Pathology and Biotechnology ; Faculty of Agriculture; Kochi University ; Nankoku , Japan.
出版信息
Plant Signal Behav. 2015;10(6):e970410. doi: 10.4161/15592316.2014.970410.
Abstract
Ralstonia solanacearum is the causal agent of bacterial wilt disease. To better understand the molecular mechanisms involved in interaction between Nicotiana benthamiana and R. solanacearum, we focused on Hsp90, RAR1 and SGT1. Appearances of wilt symptom were significantly suppressed in Hsp90, RAR1 and SGT1-silenced plants compared with control plants. In RAR1-silenced plants, population of R. solanacearum increased in a similar manner to control plants. In contrast, multiplication of R. solanacearum was significantly suppressed in Hsp90 and SGT1-silenced plants. In addition, expression of PR genes were increased in Hsp90 and SGT1-silenced plants challenged with R. solanacearum. Therefore, RAR1 might be required for disease development or suppression of disease tolerance. These results also suggested that Hsp90 and/or SGT1 might play an important role in suppression of plant defenses leading to disease susceptibility and disease development.
摘要
青枯雷尔氏菌是细菌性枯萎病的病原体。为了更好地理解本氏烟草与青枯雷尔氏菌相互作用所涉及的分子机制，我们重点研究了热休克蛋白90（Hsp90）、抗病相关蛋白1（RAR1）和SGT1。与对照植株相比，Hsp90、RAR1和SGT1基因沉默的植株中枯萎症状的出现明显受到抑制。在RAR1基因沉默的植株中，青枯雷尔氏菌的数量与对照植株以相似的方式增加。相比之下，在Hsp90和SGT1基因沉默的植株中，青枯雷尔氏菌的繁殖受到显著抑制。此外，在用青枯雷尔氏菌攻击的Hsp90和SGT1基因沉默的植株中，病程相关（PR）基因的表达增加。因此，RAR1可能是疾病发展或抑制疾病耐受性所必需的。这些结果还表明，Hsp90和/或SGT1可能在抑制植物防御导致疾病易感性和疾病发展方面发挥重要作用。
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本文引用的文献
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Plant J. 2014 Aug;79(3):427-39. doi: 10.1111/tpj.12573. Epub 2014 Jun 30.
2
Pepper suppressor of the G2 allele of skp1 interacts with the receptor-like cytoplasmic kinase1 and type III effector AvrBsT and promotes the hypersensitive cell death response in a phosphorylation-dependent manner.
Plant Physiol. 2014 May;165(1):76-91. doi: 10.1104/pp.114.238840. Epub 2014 Mar 31.
3
The role of RAR1 in Agrobacterium-mediated plant transformation.
Plant Signal Behav. 2013 Oct;8(10):doi: 10.4161/psb.26784. doi: 10.4161/psb.26784.
4
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5
The Salmonella type III effector SspH2 specifically exploits the NLR co-chaperone activity of SGT1 to subvert immunity.
PLoS Pathog. 2013;9(7):e1003518. doi: 10.1371/journal.ppat.1003518. Epub 2013 Jul 25.
6
A translationally controlled tumor protein negatively regulates the hypersensitive response in Nicotiana benthamiana.
Plant Cell Physiol. 2013 Aug;54(8):1403-14. doi: 10.1093/pcp/pct090. Epub 2013 Jun 20.
7
SGT1 contributes to coronatine signaling and Pseudomonas syringae pv. tomato disease symptom development in tomato and Arabidopsis.
New Phytol. 2011 Jan;189(1):83-93. doi: 10.1111/j.1469-8137.2010.03470.x. Epub 2010 Sep 20.
8
SGT1 positively regulates the process of plant cell death during both compatible and incompatible plant-pathogen interactions.
Mol Plant Pathol. 2010 Sep;11(5):597-611. doi: 10.1111/j.1364-3703.2010.00631.x.
9
Pseudomonas syringae hijacks plant stress chaperone machinery for virulence.
Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):13177-82. doi: 10.1073/pnas.0910943107. Epub 2010 Jul 6.
10
Cytosolic HSP90 and HSP70 are essential components of INF1-mediated hypersensitive response and non-host resistance to Pseudomonas cichorii in Nicotiana benthamiana.
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